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目的评价艾滋病病毒(HIV)抗体检测替代策略在各类人群中检测HIV抗体的可靠性,探讨HIV抗体替代策略在全人群中推广的可行性。方法对1 040份筛查阳性的样本,用6种不同的快速试剂、1种酶联免疫吸附试验(ELISA)试剂及蛋白印迹法(WB)同时进行检测,对WB不确定样本做病毒载量(VL)检测,对检测结果进行比较。结果 1 040份筛查阳性样本中,WB检测结果为阳性的有1 007份,当2种快速试剂检测结果为阳性,ELISA试剂检测结果 S/CO≥1时,一致性为100%;WB检测结果为不确定,且VL检测结果为阳性的有20份,当2种快速试剂检测结果为阳性,ELISA试剂检测结果 S/CO≥6时,一致性为100%;以上结果在各类人群中均一致。结论当2种快速试剂检测结果为阳性,且ELISA试剂检测结果 S/CO≥6时,与WB或VL检测结果一致性达到100%,可以替代WB确证检测。在保证检测试剂质量的情况下,将HIV抗体检测替代策略向全人群推广应用是可行的。
Objective To evaluate the reliability of detecting HIV antibody in all kinds of people and to explore the feasibility of using HIV antibody replacement strategy to promote the whole population. Methods A total of 1 040 samples with positive screening were tested simultaneously by six different rapid reagents, one ELISA reagent and Western blotting (WB), and the viral load was determined on WB uncertain samples (VL) test, the test results were compared. Results In 1 040 screening positive samples, there were 1 007 positive WB test results, 100 positive test results when two fast reagents were positive and ELISA reagent test results S / CO ≧ 1. The WB test The results were uncertain and VL test results were positive 20, when the two rapid reagent test results were positive, ELISA reagent test results S / CO ≥ 6, the consistency was 100%; The above results in various types of people Are the same. Conclusion When the results of two rapid reagents are positive and the results of ELISA test S / CO≥6, the consistency with WB or VL test results can reach 100%, which can replace WB confirmation test. In the case of ensuring the quality of test reagents, it is feasible to popularize and apply the HIV antibody testing alternative strategy to the whole population.