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目的：探讨层粘连蛋白总糖肽（ＬＮ－Ｇｐｓ）增强巨噬细胞（Ｍφ）杀伤黑色素瘤Ｂ１６－ＭＢＫ细胞作用的机制。方法：将细菌脂多糖（ＬＰＳ，５ＥＵ／ｍｌ），甘露聚糖（Ｍａｎｎａｎ，１０ｍｇ／ｍｌ），ＬＮ－Ｇｐｓ（２００μｇ／ｍｌ）及ＰＢＳ（对照），１００μｌ／孔，分别刺激小鼠腹腔Ｍφ，并于持续刺激６，８，１６，２４，３２，４０ｈ后观察Ｍφ对Ｂ１６细胞的直接杀伤作用及其条件培养基对Ｂ１６细胞的杀伤作用。结果：Ｍφ经各种刺激作用１６ｈ后，对Ｂ１６细胞总的杀伤率各组无显著差异；各组在刺激后各时间里Ｍφ对Ｂ１６细胞的直接杀伤率的动态变化相似。但各种刺激时间后收集的条件培养基对Ｂ１６细胞的杀伤率动态曲线明显不同，ＬＰＳ及Ｍａｎｎａｎ刺激后，以６ｈ的条件培养基杀伤作用最强（Ｐ＜０．０５），８ｈ后回到对照水平，而ＬＮ－Ｇｐｓ组则在刺激１６ｈ后逐渐增强，４０ｈ达到高峰。结论：ＬＮ－Ｇｐｓ可刺激Ｍφ分泌一些可溶性物质杀伤Ｂ１６细胞，此作用机制与其所含高甘露糖型糖链结构无关，与ＬＰＳ的作用也明显不同。
Objective: To investigate the mechanism of LN-Gps enhance macrophage (Mφ) killing melanoma B16-MBK cells. METHODS: Bacterial lipopolysaccharide (LPS, 5 EU/ml), Mannan (Mannan, 10 mg/ml), LN-Gps (200 μg/ml) and PBS (control), 100 μl/well were used to stimulate Mφ in the abdominal cavity of mice, respectively. The direct killing effect of Mφ on B16 cells and the killing effect of conditioned medium on B16 cells were observed after 6, 8, 16, 24, 32, 40 h of continuous stimulation. RESULTS: There was no significant difference in the total killing rate of B16 cells after Mφ was stimulated for 16 h. The dynamic changes of direct killing rate of Mφ to B16 cells in each group were similar at all times after stimulation. However, the dynamic curves of the killing rate of B16 cells in the conditioned medium collected after various stimulation times were significantly different. After stimulation with LPS and Mannan, the killing effect was the strongest under the condition of 6 h (P<0.05), and returned after 8 h. Control levels, while LN-Gps group gradually increased after 16h stimulation, peaked at 40h. Conclusion: LN-Gps can stimulate Mφ to secrete some soluble substances to kill B16 cells. This mechanism is not related to the structure of high mannose-type sugar chains, and it is also different from LPS.