The emergence of mobile Tigecycline-resistant tet(X3) and tet(X4) is believed to be a global threat to public health. Here, we investigated the prevalence of tet(X3) and tet(X4) in our metagenomic data of migratory birds. While tet (X4) was not identified in our samples, tet(X3) was found in two gut microbiomes of bird fecal samples, with 100% amino acid identity of sites 150–387. These results suggest that tet(X3) has been spreading into the environment for a long period of time and that there is an urgent need to control its further transmission.