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目的探讨先兆子痫胎盘组织中细胞命运决定因子numb的表达情况及意义。方法获取正常妊娠胎盘组织以及先兆子痫胎盘组织,分别运用实时荧光定量PCR法、免疫印迹法、免疫组织化学法检测numb mRNA、蛋白分布及其表达水平。结果 numb在以上两种胎盘组织中均有表达,且主要集中在细胞膜上表达。numb在先兆子痫组(实验组)的表达水平明显高于正常胎盘组(对照组)(P<0.05)。结论 numb参与的滋养细胞凋亡失调可能是先兆子痫的发病机制之一。
Objective To investigate the expression and significance of cell fate determinant numb in preeclamptic placenta. Methods Normal placenta and placenta preeclampsia were obtained. The mRNA and protein levels of numb were detected by real-time fluorescence quantitative PCR, immunoblotting and immunohistochemistry respectively. Results numb was expressed in both of the above placenta tissues and mainly concentrated on the cell membrane. The expression level of numb in preeclampsia group (experimental group) was significantly higher than that in normal placenta group (control group) (P <0.05). Conclusion The imbalance of trophoblast apoptosis involved in numb may be one of the pathogenesis of preeclampsia.