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背景:体温对脑干听觉诱发电位有显著影响,其波潜伏期是反映体温影响突触传递和神经纤维传导的客观灵敏指标。耳蜗电图波的起源有待明确,体温对耳蜗电图的影响有待探讨。目的:观察体温对耳蜗电图的影响效应,为明确耳蜗电图波的起源及指导其临床应用提供重要实验依据。设计:随机区组设计。材料:实验于2002-07/09在暨南大学医学院生理学教研室进行。取成年豚鼠40只,随机分为体温降低组和体温升高组两组,每组20只。干预:采用体表物理降温或升温法逐步降低或升高豚鼠体温,体温变化率控制在每5～10min降低或升高1℃。体温每变化1℃测试1次脑干听觉诱发电位和耳蜗电图。主要观察指标:脑干听觉诱发电位和耳蜗电图的波峰潜伏期与波峰间潜伏期。结果:37只豚鼠进入结果分析。①随体温逐步降低(36～25℃)或逐步升高(36～42℃),脑干听觉诱发电位的Ⅰ,Ⅱ,Ⅲ,Ⅳ波波峰潜伏期和Ⅰ～Ⅳ波波峰间潜伏期逐渐延长或缩短,体温越低或越高,延长或缩短越显著;耳蜗电图的N1,N2,N3波波峰潜伏期和N1～N3波波峰间潜伏期同样也随体温降低或升高而逐渐延长或缩短,体温越低或越高,延长或缩短越显著。②随体温变化,代表脑干中枢传递时间的脑干听觉诱发电位的Ⅰ～Ⅳ波波峰间潜伏期的延长总值和缩短总值明显大于代表外周传导时间的Ⅰ波波峰潜伏期的变化值,且源自脑干中枢的Ⅱ,Ⅲ,Ⅳ波的波峰潜伏期的延长总值和缩短总值明显大于源自听神经的Ⅰ波波峰潜伏期的变化值,耳蜗电图的N1～N3波波峰间潜伏期的延长总值和缩短总值也明显大于代表外周传导时间的N1波波峰潜伏期的变化值,N2,N3波波峰潜伏期的延长总值和缩短总值也明显大于源自听神经的N1波波峰潜伏期的变化值。结论:体温对耳蜗电图的显著影响与脑干听觉诱发电位相似,耳蜗电图的N2,N3波较源自听神经的N1波有更高位的神经起源。
BACKGROUND: Body temperature has a significant effect on the brainstem auditory evoked potentials, and its latency is an objective and sensitive index reflecting the effect of body temperature on synaptic transmission and nerve fiber conduction. The origin of cochlear electrophorogram needs to be clear, the impact of body temperature on the cochlear electrogram needs to be explored. Objective: To observe the effect of body temperature on the electrocochleogram and provide important experimental evidence for clarifying the origin of the electrocochlear wave and guiding its clinical application. Design: Random block design. MATERIALS: The experiment was performed at the Department of Physiology, Jinan University Medical College from July to December 09, 2002. Forty adult guinea pigs were randomly divided into two groups: hypothermia group and hyperthermia group, with 20 rats in each group. Intervention: Body surface physical cooling or warming method to gradually reduce or increase the guinea pig body temperature, rate of change of body temperature control every 5 ~ 10min reduce or increase 1 ℃. The brainstem auditory evoked potentials and the cochlear electrogram were tested for each 1 ° C change in body temperature. MAIN OUTCOME MEASURES: Peak latency and peak latency of brainstem auditory evoked potentials and cochlear electrograms. Results: 37 guinea pigs entered the result analysis. (1) The peak latency of Ⅰ, Ⅱ, Ⅲ, Ⅳ wave and the latency of Ⅰ ~ Ⅳ wave peak gradually lengthened or shortened as the body temperature decreased gradually (36 ~ 25 ℃) or increased gradually (36 ~ 42 ℃) , The lower or the higher the body temperature, the more obvious the prolongation or the shortening. The peak latency of N1, N2, N3 wave and the latency of N1 ~ N3 wave in the ECG also prolong or shorten with the decrease or increase of body temperature. Lower or higher, the more significant extension or shortening. (2) With the changes of body temperature, the total and shortening of the latency of Ⅰ ~ Ⅳ wave peak of brainstem auditory evoked potentials, which represent the time of brain stem transit, were significantly greater than that of Ⅰ wave peak, which represents the peripheral conduction time, The total and shortening peak values of wave latency of Ⅱ, Ⅲ and Ⅳ waves from the brainstem center were significantly larger than those of the I wave from the auditory nerve, and the prolongation of the latency between wave peaks of N1 ~ N3 wave of the ECG The total values of shortening and shortening were also significantly larger than those of the N1 wave representing the conduction time. The total and shortening values of the N1 and N3 wave peaks were significantly higher than that of the N1 wave from the auditory nerve. CONCLUSION: The significant effect of body temperature on ECG is similar to that of brainstem auditory evoked potentials. The N2 and N3 waves of ECG have higher neurogenic origin than the N1 wave originated from auditory nerve.