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目的探讨乙肝病毒表面抗原(HBsAg)浓度与α-L-岩藻糖苷酶(AFU)及甲胎蛋白(AFP)的相关性。方法收集240例HBsAg阳性病例的血清,根据HBsAg定量的不同浓度分为1组(0.5ng/ml～150ng/ml)、2组(151ng/ml～300ng/ml)、3组(301ng/ml～450ng/ml)、4组(>450ng/ml),每组60例,正常对照组60例,分别检测AFU、AFP,对其结果进行分析。AFU检测采用速率法,HBsAg检测采用时间分辨免疫荧光法,AFP检测采用化学发光法。结果1组的AFU与AFP呈正相关(r=0.310,P<0.05),2组的AFU与AFP也呈正相关(r=0.298,P<0.05),3组和4组的AFU与AFP之间无相关性(分别r=0.298,r=0.067,P>0.05);各HBsAg浓度组与对照组AFU比较差异无统计学意义(F=1.719,P>0.05),而与对照组AFP比较差异有统计学意义(F=3.000,P<0.05);各组AFU阳性率随HBsAg浓度升高无升高趋势(χ2=1.44,P>0.05),而AFP阳性率随HBsAg浓度升高呈升高趋势(χ2=8.28,P<0.005)。结论对HBsAg高浓度的HBV感染者同时检测AFP和AFU对观察肝脏损害的演变过程有重要的临床价值。
Objective To investigate the relationship between HBsAg concentration and alpha-L-fucosidase (AFU) and alpha-fetoprotein (AFP). Methods Serum from 240 HBsAg positive cases were collected and divided into 1 group (0.5ng / ml ~ 150ng / ml), 2 groups (151ng / ml ~ 300ng / ml) and 3 groups 450ng / ml), 4 groups (> 450ng / ml), 60 cases in each group and 60 cases in normal control group. AFU and AFP were detected respectively and the results were analyzed. AFU detection rate method, HBsAg detection using time-resolved immunofluorescence, AFP detection using chemiluminescence. Results There was a positive correlation between AFU and AFP in group 1 (r = 0.310, P <0.05). There was also a positive correlation between AFU and AFP in group 2 (r = 0.298, P <0.05) (R = 0.298, r = 0.067, P> 0.05). There was no significant difference in AFU between HBsAg concentration group and control group (F = 1.719, P> 0.05) (Χ2 = 1.44, P> 0.05). However, the positive rate of AFP increased with the increase of HBsAg concentration (χ2 = 1.44, P> 0.05) 8.28, P <0.005). Conclusions Simultaneous detection of AFP and AFU in HBV infected patients with high HBsAg levels has important clinical value in the observation of the evolution of liver damage.