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目的研究喇叭藻多糖对α-葡萄糖苷酶抑制活性。方法采用热水提取从拟小叶喇叭藻(Turbinaria conoides)和喇叭藻(Turbinaria ornata)中获得水溶性多糖TCP和TOP,并采用硫酸-苯酚法测定其总糖含量;用改良的咔唑法测定其糖醛酸含量;用Folin-酚法(Lowry法)测定其蛋白含量;用高效液相色谱法(HPLC)测定其单糖组成;用离子色谱法(IC)测定其硫酸根含量;用高效凝胶渗透色谱与多角度激光散射仪联用法(HPGPC-MALLS)测定其平均分子量;用PNPG显色法测定TCP和TOP对α-葡萄糖苷酶抑制活性。结果 TCP和TOP具有良好的α-葡萄糖苷酶抑制活性,IC50值分别为204.1和113.2μg·mL~(-1),效果优于阿卡波糖(220.3μg·mL~(-1))。初步动力学研究表明其均为混合型抑制方式。结论热水提取自拟小叶喇叭藻和喇叭藻的多糖具有良好的α-葡萄糖苷酶抑制活性。
Objective To study the inhibitory activity of polysaccharide from hornweed on α-glucosidase. Methods The water-soluble polysaccharides TCP and TOP were extracted from Turbinaria conoides and Turbinaria ornata by hot water extraction. The total sugar content was determined by sulfuric acid-phenol method. The content of total sugar was determined by modified carbazole method The content of uronic acid was determined by Folin-phenol method (Lowry method). The monosaccharide composition was determined by high performance liquid chromatography (HPLC). The sulfate content was determined by ion chromatography (IC) The average molecular weight was determined by gel permeation chromatography (GPC) and multi-angle laser light scattering (HPGPC-MALLS). The inhibitory activity of TCP and TOP against α-glucosidase was determined by PNPG assay. Results TCP and TOP had good α-glucosidase inhibitory activity with IC50 values of 204.1 and 113.2 μg · mL -1, respectively, which was superior to that of acarbose (220.3 μg · mL -1). Preliminary kinetic studies show that they are mixed suppression. Conclusion The polysaccharides extracted by hot water from Brassica lobophylla and Brassica alba have good α-glucosidase inhibitory activity.