论文部分内容阅读
目的:探讨p53、CerbB2和nm23基因在非小细胞肺癌(NSCLC)组织中表达的临床意义。方法:组织学或细胞学证实的NSCLC89例,采用免疫组化法检测p53、CerbB2和nm23蛋白表达,并取癌旁正常肺组织87例作为对照。结果:肺癌组织p53和CerbB2蛋白表达率分别为60%和67%,均高于正常肺组织5%和3%(P<0.05);nm23蛋白表达为67%,则显著低于正常肺组织93%(P<0.05)。p53蛋白表达与肺癌组织的病理类型、原发肿瘤范围(T)和肿瘤分化程度有显著的相关性(P<0.05),与患者的性别、年龄、疾病分期(TNM)、淋巴结受侵范围(N)和远处转移状况(M)没有明显的相关性。CerbB2蛋白表达与患者的性别、年龄、原发肿瘤大小(T)、淋巴结受侵范围(N)和远处转移状况(M)、疾病分期(TNM)及肿瘤分化程度均没有明显的相关性。nm23蛋白表达与肿瘤的淋巴结受侵范围(N)、远处转移状况(M)和疾病分期(TNM)存在显著相关性(P<0.05),与患者的性别、年龄、原发肿瘤大小(T)及分化程度没有显著相关性。结论:p53基因编码的蛋白在NSCLC表达增高及nm23基因编码的蛋白表达降低,提示p53和nm23基因在NSCLC的发生发展、病理特征和转移中可能起重要作用。
Objective: To investigate the clinical significance of p53, CerbB2 and nm23 gene expression in non-small cell lung cancer (NSCLC). METHODS: Totally 89 cases of NSCLC confirmed by histology or cytology were examined for the expression of p53, CerbB2 and nm23 by immunohistochemistry and 87 normal lung tissues adjacent to the tumor were used as controls. Results: The positive rates of p53 and CerbB2 in lung cancer tissues were 60% and 67%, respectively, which were higher than those in normal lung tissues by 5% and 3%, respectively (P <0.05). The nm23 protein expression was 67% % (P <0.05). There was a significant correlation between the expression of p53 protein and the pathological type of lung cancer, the extent of primary tumor (T) and the degree of tumor differentiation (P <0.05), but not with the gender, age, stage of disease (TNM) N) and distant metastasis (M) no significant correlation. CerbB2 protein expression had no significant correlation with patient’s gender, age, primary tumor size (T), extent of lymph node involvement (N) and distant metastasis (M), disease stage (TNM) and tumor differentiation. There was a significant correlation between the expression of nm23 protein and the extent of lymph node invasion (N), distant metastasis (M) and disease stage (TNM) (P <0.05) ) And the degree of differentiation was not significantly correlated. CONCLUSION: The increased expression of p53 gene in NSCLC and the decreased expression of nm23 gene suggest that p53 and nm23 genes may play an important role in the development of NSCLC, pathological features and metastasis.