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目的:探讨淫羊藿苷在去卵巢大鼠骨质疏松症中的保护作用及机制。方法:将55只大鼠(均为雌性,6个月龄)随机分为5组(对照组、模型对照组、西药组、淫羊藿小剂量组及大剂量组),每组均为11只。手术取出大鼠双侧卵巢以建立骨质疏松大鼠模型,14天后开始给药,西药组大鼠给予尼尔雌醇(1mg·kg~(-1))灌胃,小剂量及大剂量组分别给予淫羊藿苷(50mg·kg~(-1)、100mg·kg~(-1))灌胃,1次/天。给药84天后,进行下腹主动脉取血并处死。观察大鼠骨组织形态学、骨密度(BMD)、细胞凋亡蛋白的变化。结果:与对照组比较,模型对照组大鼠的BMD水平明显降低(P<0.01);西药组、小剂量组、大剂量组分别与模型对照组比较,BMD水平明显升高(P<0.01)。与对照组比较,模型对照组大鼠的血清钙水平明显降低,血清磷水平明显升高(P<0.05);与模型对照组比较,给予淫羊藿苷治疗的小剂量组及大剂量组大鼠血清钙水平明显升高,血清磷水平明显降低(P<0.05);与对照组比较,模型对照组大鼠的骨组织中Caspase蛋白、Bax蛋白表达水平明显上调,而Bcl-2蛋白水平明显下调,P均<0.05;与模型对照组比较,西药组、小、大剂量组大鼠骨组织中的Caspase蛋白、Bax蛋白表达明显下降,而Bcl-2蛋白水平明显升高,P均<0.05。结论:淫羊藿苷在去卵巢大鼠骨质疏松症治疗中有保护作用,且其保护机制可能和细胞凋亡蛋白受到抑制有关。
Objective: To investigate the protective effect of icariin on osteoporosis in ovariectomized rats and its mechanism. Methods: 55 rats (all female, 6 months old) were randomly divided into 5 groups (control group, model control group, Western medicine group, Epimedium low dose group and high dose group), each group was 11 only. The bilateral ovaries were removed by surgery to establish the rat model of osteoporosis. The rats were started administration 14 days later. The rats in the western medicine group were given nilestriol (1 mg · kg -1) intragastrically, and the small dose and high dose groups Icariin (50 mg · kg -1, 100 mg · kg -1) was given intragastrically once daily. After 84 days of administration, the lower abdominal aorta was taken and sacrificed. The morphological changes of bone tissue, bone mineral density (BMD) and apoptosis proteins were observed. Results: Compared with the control group, the BMD level in the model control group was significantly decreased (P <0.01); the BMD level in the western medicine group, the low-dose group and the high-dose group were significantly increased compared with the model control group (P <0.01) . Compared with the control group, the serum calcium level of the model control group was significantly decreased and the serum phosphorus level was significantly increased (P <0.05). Compared with the model control group, the low-dose and high-dose groups treated with icariin Compared with the control group, the expression of Caspase protein and Bax protein in the model control group was significantly increased, while the level of Bcl-2 protein was significantly increased (P <0.05) P <0.05). Compared with the model control group, the expression of Caspase protein and Bax protein in bone tissue of rats in small dose group and high dose group decreased significantly, while the level of Bcl-2 protein increased significantly (P <0.05) . Conclusion: Icariin has a protective effect on the treatment of osteoporosis in ovariectomized rats, and its protective mechanism may be related to the inhibition of the apoptosis protein.