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应用细胞培养技术 ,采用半定量RT PCR法和ELISA检测等方法 ,以正常人为对照 ,观察川芎嗪 (33μg/ml)和地塞米松 (10 5mol/L )对培养的狼疮肾炎 (LN )活动期、静止期外周血单个核细胞 (PBMC )表达IL 12和产生免疫球蛋白 (Ig)的影响。结果表明 ,LN活动期、静止期较正常对照组IL 12蛋白、基因水平明显增高 (P <0 0 1) ,川芎嗪仅对活动期狼疮肾炎PBMCIL 12表达明显抑制 ,而地塞米松明显抑制狼疮肾炎PBMCIL 12表达 (P <0 0 1) ,两种药物对正常人对照IL 12表达及合成Ig无明显抑制作用 (P >0 0 5 ) ,川芎嗪和地塞米松均明显抑制狼疮肾炎活动期及静止期Ig合成 (P <0 0 1或 <0 0 5 )。提示 :狼疮肾炎患者有高水平的IL 12 ,川芎嗪、地塞米松通过下调狼疮肾炎IL 12表达及Ig的合成 ,减轻狼疮肾炎的发生和发展。
The effects of ligustrazine (33 μg / ml) and dexamethasone (105 mg / ml) on the activity of lupus nephritis (LN) were detected by semi-quantitative RT-PCR and ELISA. , Resting peripheral blood mononuclear cells (PBMCs) expressing IL-12 and producing immunoglobulins (Ig). The results showed that IL 12 protein and gene levels in active and quiescent LNs were significantly increased compared with those in normal controls (P <0.01), while tetramethylpyrazine was only inhibited significantly in active lupus nephritis PBMCIL 12, while dexamethasone significantly inhibited lupus Nephritis PBMCIL 12 expression (P <0.01), the two drugs on normal control IL12 expression and synthetic Ig had no significant effect (P> 0.05), tetramethylpyrazine and dexamethasone were significantly inhibited lupus nephritis active period And quiescent Ig (P <0 0 1 or <0 0 5). Tip: Lupus nephritis patients with high levels of IL 12, ligustrazine, dexamethasone lupus nephritis by reducing the expression of IL 12 and Ig synthesis, reduce the occurrence and development of lupus nephritis.