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通过在上海崇明岛菜田设置的定位试验,采用Biolog生态板法研究生物耕作时间对不同深度土层中土壤微生物群落多样性及其碳代谢特征的影响。3年的结果表明:生物耕作显著提高了微生物群落活性,且随着耕作年限的延长增加值越高,生物耕作3年可使平均AWCD值升高3~7倍。生物耕作处理Simpson指数和Shannon指数均显著高于免耕对照,在0~5 cm土层累积增幅分别为49和6.28,5~20 cm土层累积增幅分别为31和2.55。蚯蚓生物耕作显著增加土壤微生物对6类碳源的代谢能力,耕作3年碳水化合物代谢活性大幅增加。本研究说明蚯蚓生物耕作是增加土壤微生物代谢活性的有效途径。
Biolog ecological plate method was used to study the effects of biological tillage time on soil microbial community diversity and carbon metabolism in different depths of soil layers. The results of three years showed that biological tillage significantly enhanced the microbial community activity, and the higher the added value of tillage, the average AWCD value increased by 3 to 7 times after three years of cultivation. The Simpson index and Shannon index of the biological tillage treatments were significantly higher than those of the no-tillage control. The cumulative increases in the 0-5 cm soil layer were 49 and 6.28, respectively. The cumulative increases of the soil layers in the 5-20 cm soil layer were 31 and 2.55, respectively. The biotrowling of earthworms significantly increased the metabolic capacity of soil microorganisms to 6 types of carbon sources, and the carbohydrate metabolic activity increased significantly after 3 years of farming. This study shows that earthworm bio-tillage is an effective way to increase the metabolic activity of soil microorganisms.