本文报道了一个新的鼠疫菌分离方法——双相管培养法。它的检出率比传统方法高出两倍以上,选择性强,原始材料保存与增菌同步,弱毒或无毒株也可生长,生长状态和菌落形态典型而富有特色,对非鼠疫菌还有一定的鉴别功能,操作方便,易于质控,可用于鼠疫流行静止期的大面积查源与监测,节约大量人力和资金。 This article reports a new method for the isolation of Yersinia pestis - biphasic tube culture. Its detection rate is more than twice as high as that of the traditional method, and its selectivity is high. The preservation of raw materials is synchronized with enrichment, the attenuated or non-toxic strains can grow, the growth state and colony morphology are typical and distinctive, A certain identification function, easy to operate, easy to control, can be used for large areas of epidemic quarantine and monitoring of plague, saving a lot of manpower and capital.