论文部分内容阅读
目的 探讨青年人与老年人胃癌不同临床特点的分子生物学基础。方法 对 6 5例胃癌 (其中青年组 35例 ,老年组 30例 )标本连续切片分别作增殖细胞核抗原 (PCNA)免疫组化染色及核仁组成区相关的嗜银蛋白 (AgNOR)的胶体银染色 ,前者结果进行 χ2 检验 ,后者采用t检验。结果 在非选择人群 ,PCNA的阳性表达率及AgNOR计数均随胃癌分化程度降低而提高 ,AgNOR数目还随浸润深度的增加而增多 (P <0 .0 5 )。在具有相同的组织学分化程度、浸润深度或局部淋巴结转移情况的胃癌中 ,青年组中PCNA的阳性率及AgNOR数目均显著高于老年组 (P <0 .0 5 )。 结论 PCNA及AgNOR在青年人胃癌细胞中的高表达提示青年人胃癌细胞具有更强的增殖活性及浸润转移能力。
Objective To explore the molecular biological basis of different clinical features of gastric cancer in young people and the elderly. Methods Sixty-five specimens of gastric cancer (35 in the young group and 30 in the old group) were serially sectioned for proliferating cell nuclear antigen (PCNA) immunohistochemical staining and colloidal silver staining of the nucleolar organizing region-associated AgNOR. The former produces a χ2 test and the latter uses a t test. Results In non-selected populations, the positive expression rate of PCNA and AgNOR counts increased with the decrease of differentiation of gastric cancer, and the number of AgNOR also increased with the depth of invasion (P < 0.05). In gastric cancer with the same degree of histological differentiation, depth of invasion, or local lymph node metastasis, the positive rate of PCNA and the number of AgNOR in the young group were significantly higher than those in the elderly group (P < 0.05). Conclusion The high expression of PCNA and AgNOR in young gastric cancer cells suggests that young human gastric cancer cells have stronger proliferative activity and infiltration and metastasis capacity.