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目的 研究血管紧张素Ⅰ转换酶 (ACE)基因多态性是否与 2型糖尿病肾病 (DN)相关。方法 用聚合酶链反应 (PCR)扩增ACE基因第 1 6内含子的一个 2 87bp的插入 /缺失 (I/D)基因片段 ,1 5 %琼脂糖凝胶电泳 ,紫外线灯下观察结果。共检测了 2 2 5例江苏籍汉人 (2型糖尿病 1 75例 ,其中 1 0 0例不伴DN ,75例伴DN ;对照者 5 0例 )的ACEI/D基因型。结果 各组间ACEI/D基因型和等位基因频率分布无明显差异 (P >0 0 5 )。结论 ACE基因I/D多态性与 2型DN的发病无明显相关性。
Objective To investigate whether the polymorphism of angiotensin Ⅰ converting enzyme (ACE) is associated with type 2 diabetic nephropathy (DN). Methods A 2 87 bp insertion / deletion (I / D) gene fragment was amplified by polymerase chain reaction (PCR) from the 16th intron of ACE gene. The results were observed under 1% UV agarose gel electrophoresis. ACEI / D genotypes were detected in 225 Chinese Han nationality in Jiangsu Province (175 cases of type 2 diabetes, 100 cases without DN, 75 cases with DN and 50 cases with control). Results There was no significant difference in the frequencies of ACEI / D genotypes and alleles between groups (P> 0.05). Conclusion There is no significant correlation between ACE gene I / D polymorphism and the incidence of type 2 DN.