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目的观察静脉移植骨髓间充质干细胞(BMSCs)对大鼠脑梗死的治疗作用并探讨其对凋亡蛋白的影响。方法全骨髓培养法分离纯化大鼠BMSCs。改良Longa线栓法成功建立大鼠脑梗死模型45只,随机分为空白组、对照组和实验组,建模1d后,空白组无处理因素,对照组尾静脉注射1m L生理盐水,实验组注射1m L BMSCs悬液。在建模后当日、BMSCs移植后第3、7d进行神经功能评分测试(m NSS),移植后第7d进行TTC法检测各组脑梗死体积,同时检测脑区Brd U阳性细胞,用免疫组化及Western blot检测Bcl-2、caspase-3蛋白表达。结果 m NSS显示,移植前3组得分差异无统计学意义,移植后细胞移植组得分较对照组及空白组明显降低(P<0.01),且空白组与对照组差异无统计学意义(P>0.05);TTC检测显示,细胞移植组较其他2组梗死体积明显减小(P<0.05),空白组与对照组梗死体积差异无统计学意义(P>0.05);移植组梗死脑区有Brd U阳性细胞;免疫组化显示,细胞移植组较其他2组Bcl-2阳性细胞明显增高(P<0.01),caspase-3阳性细胞明显降低(P<0.05),且空白组与对照组差异无统计学意义(P>0.05);western显示,细胞移植组较其他2组Bcl-2蛋白表达明显增加(P<0.01),caspase-3蛋白表达明显降低(P<0.05),且空白组与对照组差异无统计学意义(P>0.05)。结论静脉移植BMSCs可改善梗死大鼠的神经功能,其对脑梗死的治疗作用与其抑制凋亡作用有关。
Objective To observe the therapeutic effect of intravenous transplanted bone marrow mesenchymal stem cells (BMSCs) on cerebral infarction in rats and its effect on apoptosis protein. Methods BMSCs were isolated and purified by whole bone marrow culture. Forty-five rats were randomly divided into blank group, control group and experimental group. After modeling for 1 day, blank control group received no treatment and control group received intravenous injection of 1 ml normal saline. The experimental group Inject 1 mL of BMSCs suspension. On the day after modeling, neurological function score (m NSS) was measured on the 3rd and 7th day after transplantation. At 7th day after transplantation, the volume of cerebral infarction was detected by TTC method. BrdU positive cells were detected by immunohistochemistry Western blot was used to detect the protein expression of Bcl-2 and caspase-3. Results The results of m NSS showed that there was no significant difference between the three groups before transplantation, the cell transplantation group after transplantation was significantly lower than that of the control group and the blank group (P <0.01), and there was no significant difference between the blank group and the control group (P> 0.05). TTC showed that the infarct volume of the cell transplantation group was significantly lower than that of the other two groups (P <0.05), but there was no significant difference between the blank group and the control group (P> 0.05) U positive cells. Immunohistochemistry showed that the number of Bcl-2 positive cells in the cell transplantation group was significantly higher than that in the other two groups (P <0.01), the number of caspase-3 positive cells was significantly decreased (P <0.05), and there was no difference between the blank group and the control group Western blot showed that the expression of Bcl-2 protein in the cell transplantation group was significantly increased (P <0.01) and the expression of caspase-3 protein was significantly decreased in the cell transplantation group compared with the control group (P <0.05) There was no significant difference between the two groups (P> 0.05). Conclusion Intravenous transplantation of BMSCs can improve the neurological function of infarcted rats, and its therapeutic effect on cerebral infarction is related to the inhibition of apoptosis.