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为明确被微囊藻毒素(MCs)污染的灌溉水对农业生产的潜在危害,采用水培法研究了不同浓度(1、100、1000、3000μg·L-1)MCs处理对胁迫期和恢复期内水稻叶片MCs积累量、叶绿素含量和叶绿素荧光参数的影响.结果表明,胁迫处理7 d后,MCs在叶片中的积累量随MCs处理浓度的增大而升高.与CK相比,1μg·L-1MCs处理组水稻叶片的各生长指标和叶绿素含量上升,F0下降,Fv/Fm、ETR、q P和q N均未发生显著变化;高浓度(≥100μg·L-1)处理下,水稻叶片的生长受抑制,叶绿素含量下降,F0上升,Fv/Fm、ETR、q P和q N显著下降.恢复7 d后各处理组水稻叶片MCs的积累量均低于胁迫期,100μg·L-1MCs处理组的F0、q N均接近CK,Fv/Fm、ETR和q P虽低于CK却高于胁迫期,表明MCs对光合系统的伤害有一定程度的恢复.1000和3000μg·L-1MCs处理组的F0依然高于CK,且Fv/Fm、ETR、q P和q N不仅低于CK也低于胁迫期,表明高浓度MCs(≥1000μg·L-1)降低了水稻叶片PSⅡ原初光能转换效率和PSⅡ潜在活性,对叶片光合功能造成不可逆的伤害.
In order to clarify the potential harm of irrigation water contaminated with microcystin (MCs) to agricultural production, hydroponics was used to study the effects of MCs at different concentrations (1, 100, 1000, 3000 μg · L-1) The content of MCs, chlorophyll content and chlorophyll fluorescence parameters in rice leaves were studied.The results showed that the accumulation of MCs in leaves increased with the increase of MCs concentration after 7 days of stress treatment.Compared with CK, 1μg · The growth index and the content of chlorophyll in leaves of L-1MCs treatment group increased, F0 decreased, while Fv / Fm, ETR, qP and qN did not change significantly. Under high concentration (> 100μg · L-1) Fv / Fm, ETR, q P and q N decreased significantly.After 7 days of recovery, the accumulation of MCs in leaves of all treatments were lower than that of stress, 100 μg · L- F0 and qN of 1MCs treatment group were close to CK, Fv / Fm, ETR and qP were lower than CK but higher than the stress period, indicating that the damage of MCs to the photosynthetic system to a certain extent .1000 and 3000μg · L-1MCs F0 in treatment group was still higher than that in CK, and Fv / Fm, ETR, q P and q N were not only lower than CK but also lower than stress period, indicating that high concentrations of MCs (≥1000μg · L -1) Reduced the primary light energy conversion efficiency of PSII and the potential activity of PSII in rice leaves, causing irreversible damage to the photosynthetic function of the leaves.