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目的 探讨WT1基因在白血病中的作用。方法 用定量RT -PCR方法检测病人外周血或骨髓单个核细胞WT1mRNA表达。结果 5 0例白血病患者中 ,41例WT1mRNA表达增高 ,阳性率为 82 0 %。急性髓细胞白血病 (AML)和急性淋巴细胞白血病 (ALL)阳性率分别为 84 0 %和 93 8% ,两组无显著差异 (P >0 0 5 ) ,慢性粒细胞白血病 (CML)的WT1mRNA阳性率为 6 6 7% ,其中慢性期均阴性 ,加速期和急变期均阳性。 17例完全缓解患者中 ,15例 (85 2 % )WT1mRNA转阴 ,复发患者WT1mRNA再次升高。结论 WT1mRNA表达的测定对白血病微小残留病检测具有重要意义
Objective To investigate the role of WT1 gene in leukemia. Methods Quantitative RT-PCR was used to detect the expression of WT1 mRNA in peripheral blood or bone marrow mononuclear cells. Results Among the 50 leukemia patients, the expression of WT1 mRNA was increased in 41 patients, the positive rate was 82%. The positive rates of acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) were 84.0% and 93.8%, respectively, and there was no significant difference between the two groups (P > 0.05). WT1 mRNA positive for chronic myeloid leukemia (CML) was positive. The rate was 66.7%, of which the chronic phase was negative, and both the acceleration phase and the blast phase were positive. Of the 17 patients with complete remission, 15 (85 2%) of the WT1 mRNA was negative, and the WT1 mRNA was re-elevated in the relapsed patients. Conclusion The detection of WT1 mRNA expression is of significance in the detection of minimal residual disease in leukemia.