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目的 采用顺序特异引物聚合酶链反应 (PCR -SSP)建立人类白细胞抗原DR位点的DNA分型方法 .方法 合成 2 9个特异性引物和 1对阳性对照引物 ,组成 2 0个PCR反应用于DR位点 ,建立一步法PCR -SSP .结果 所有样本PCR -SSP基因分型获得成功 ,分型结果经标准DNA ,限制性核酸内切酶分析证实符合 ,特异性和重复性 10 0 % .结论 PCR -SSP检测HLA -DR的方法具有快速、准确、特异性高等优点 ,适合临床应用 .
OBJECTIVE: To establish a DNA typing method for human leukocyte antigen (DR) locus using sequence-specific primer polymerase chain reaction (PCR-SSP) method.Methods Twenty-nine specific primers and one pair of positive control primers were synthesized and used to make 20 PCR reactions DR site and establish a one-step PCR-SP method.Results All the samples were successfully genotyped by PCR-SSP, and the typing results were confirmed by standard DNA and restriction endonuclease analysis to be consistent, specific and reproducible.100% .Conclusion PCR -SSP detection of HLA-DR method has the advantages of fast, accurate, high specificity, suitable for clinical applications.