Are nuclear loci ideal for barcoding plants? A case study of genetic delimitation of two sister spec

来源 :植物分类学报 | 被引量 : 0次 | 上传用户:jyk7978610
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Considerable debate remains as to which DNA region should be used to barcode plants. Several different chloroplast (cp) DNA regions (rbcL, matK, and trnH-psbA) and nuclear ribosomal internal transcribed regions (ITS) have been suggested as suitable barcodes in plants. Recently, low-copy nuclear loci were also suggested to be potentially ideal barcode regions. The aim of the present study was to test the effectiveness of these proposed DNA fragments and five additional low-copy loci (CHS, DETl, COPl, PGICl, and RPS2; comprising both coding and non-coding regions) in barcoding closely related species. We examined the divergences within and between two species of Pugioniun (Brassicaceae). We failed to find any interspecific variation from three cpDNA fragments with which to discriminate the two species. However, a single base mutation in the internal transcribed spacer (ITS) could discriminate between the two species consistently. We found more variations among all individuals of the two species using each of the other five low-copy nuclear loci. However, only alleles from one locus (DET1) of the five low-copy loci related to flowering regulations was able to distinguish the sampled individuals into two species. We failed to amplify the corresponding fragments out of Brassicaceae using the designed DETl primers. We further discussed the discrimination power of different loci due to incomplete lineage sorting, gene flow, and species-specific evolution. Our results highlight the possibility of using the nuclear ITS as a core or complementary fragment to barcode recent diverged species.
其他文献
Modern petascale and future exascale systems are massively heterogeneous architectures. Developing productive intra-node programming models is crucial toward ad
Transmission electron microscopy (TEM) and physics-chemical phase analysis were employed to investigate the precipitates in high strength steels microalloyed wi
In this paper new high-strength and high-plasticity twinning induced plasticity (TWIP) steel for modern automobile body was investigated. Some basic experimenta
The dissolution of petroleum asphaltenes with ionic liquids is studied for the first time. The results show that the ionic liquids could be used as novel solven
Blending is an important unit operation in process industry. Blending scheduling is nonlinear optimization problem with constraints. It is difficult to obtain o
Microstructure and mechanical properties of pure magnesium and AZ31 alloy with Ca/Si based refiner addition were investigated. The results indicate that additio
Archives of threaded discussions generated by users in online forums and discussion boards contain valuable knowledge on various topics. However, not all thread
通过对高电压直流输电(HVDC)换流阀控制原理的分析,在目前国内智能电网的发展现状和国际上现场可编程器件(FPGA)最新技术的基础上,提出了一种基于部分重配置技术的HVDC换流阀
2011年6月,国家能源局副局长钱智民表示,“十二五”期间,中国将进入城镇化和城市发展双重转型的新阶段。在城市规划和建设中,中国应贯彻低碳理念,着力推进交通、建筑和照明节能,推
为了从广泛使用的智能卡付费系统获取公交客流信息,提出了一种利用公交调度信息和智能卡刷卡信息推断乘客上车站点的方法.对同一辆车的连续2次刷卡进行朴素贝叶斯分类,区分是