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目的 探讨日本血吸虫TPI DNA疫苗对血吸虫肝脏虫卵肉芽肿的免疫调节作用,以评价其作为抗病疫苗的潜能。方法 30头松江猪分为3组。A组(TPI组)每头猪于两侧臀部肌注500μgpcDNA3.1-SjCTPI质粒DNA;B组(TPI+IL-12组)每头猪肌注500μg pcDNA3.1-SjCTPI DNA疫苗及500μg pcDNA3.1-P35和500μg pcDNA3.1-P40质粒DNA的混和物;C组(对照组),每头猪肌注500μg pcDNA3.1质粒DNA。于第0、3、6周共免疫3次。末次免疫后30d,每头猪采用背部贴片法攻击感染600条日本血吸虫尾蚴,攻击后45 d剖杀实验猪,取肝脏,于同一部位切取1.5 cm~3的肝组织。按常规法制备石蜡切片。切片在NYD-1000型图像分析系统下观察,测定所有肉芽肿的面积和直径,并比较。结果 与对照组相比TPI组及TPI+IL-12组肉芽肿内炎性细胞明显减少。肉芽肿平均面积较对照组分别减少41.95%和42.50%,肉芽肿直径分别减少25.14%和25.66%。结论 SjCTPI DNA疫苗具有下调血吸虫肝脏肉芽肿的作用,可望成为一种血吸虫病抗病疫苗。
Objective To investigate the immunomodulatory effect of the TPI DNA vaccine of Schistosoma japonicum on liver granuloma of liver in schistosomiasis so as to evaluate its potential as a disease-resistant vaccine. Methods Thirty Songjiang pigs were divided into three groups. A group (TPI group), 500μg pcDNA3.1-SjCTPI plasmid DNA was intramuscularly injected into the buttocks of each pig. 500μg pcDNA3.1-SjCTPI DNA vaccine and 500μg pcDNA3 were intramuscularly injected into each group in group B (TPI + IL-12). 1-P35 and 500μg pcDNA3.1-P40 plasmid DNA mixture; Group C (control group), each pig intramuscular injection of 500μg pcDNA3.1 plasmid DNA. Three immunizations were given at weeks 0, 3 and 6. At the end of the last immunization, 600 pigs were infected with cercariae of Schistosoma japonicum by dorsal patch method. The experimental pigs were sacrificed 45 days after the challenge. The liver was harvested and the liver tissue was cut at the same site for 1.5 cm ~ 3. Paraffin sections were prepared by conventional methods. The sections were observed under the NYD-1000 image analysis system and the area and the diameter of all granulomas were determined and compared. Results Compared with the control group, inflammatory cells in granuloma in TPI group and TPI + IL-12 group were significantly decreased. The average area of granuloma was reduced by 41.95% and 42.50% respectively compared with the control group, while the diameter of granuloma decreased by 25.14% and 25.66% respectively. Conclusion SjCTPI DNA vaccine has the effect of down-regulating schistosomal liver granuloma and is expected to become a disease-resistant vaccine against schistosomiasis.