Objective: This study aimed to develop Nurses and Midwives’ Perceptions of their Roles in Primary Healthcare (NMPR-PHC) and evaluate its psychometric properties.Methods: A cross-sectional survey was performed to recruit a convenient sample of 150 registered nurses and midwives from various primary healthcare settings in Jordan.Reliability was evaluated by examining the intal consistency and split-half reliability of the item.A exploratory factor analysis was performed to assess the factor structure of the NMPR-PHC.Results: The final version of NMPR-PHC contained 18 items.Exploratory factor analysis revealed six factors (care coordination and interprofessional collaboration,workplace facilitators of the primary healthcare,management of care,research,workplace constraints of primary healthcare,and advanced education) for the questionnaire which explained 66.49％ of the total variance.The Cronbach’s α of the total scale was 0.834,the subscales Cronbach’s α were ranging between 0.662 and 0.770,and the splithalf reliability of the total scale was 0.734.Conclusion: The overall performance of the questionnaire showed promising sound psychometric properties.The NMPR-PHC can be recommended for use as a tool for the assessment of nurses and midwives’ perceptions of their roles in primary healthcare.