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曾有人报道人的粪便中含有致突变剂,且多数致突变剂亦为致癌剂,因此粪便中的致突变剂可能是结肠癌的病因。流行病学研究也指出,进食低肉类、高纤维素膳食的人群结肠癌发病率低。为此,作者采用彷徨变异测验(Fluctuation test)比较了素食人群与非素食人群粪便中致突变剂的情况,其灵敏度可达标准Ames法的100倍。彷徨变异测验系采用TA100与TA 98两个菌株。先将试验用菌株接种于营养肉汤中培养过夜,再用营养肉汤稀释为
It has been reported that human excrement contains mutagens, and most mutagens are also carcinogens, so mutagens in the stool may be the cause of colon cancer. Epidemiological studies also pointed out that low-fat, high-fiber diet in people with low incidence of colon cancer. To this end, the authors compared mutagenic agents in faeces between vegetarians and non-vegetarians using a Fluctuation Test that was 100 times more sensitive than the standard Ames method. Imitation 徨 mutation test using TA100 and TA 98 two strains. First test strains were inoculated in nutrient broth culture overnight, and then diluted with nutrient broth as