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基于高等植物叶绿素吸收光谱的强弱差异性,分别采用波长(442±9)nm蓝光、(457±7)nm蓝光、(521±14)nm绿光、(627±7)nm红光和(655±11)nm红光光谱发光二极管(LED)光源,以300μmol·m~(-2)·s~(-1)光强对叶用莴苣进行12 h·d~(-1)照射处理3周,以温室自然光为对照,研究具有一定能量供应下叶用莴苣生长和营养累积分配的光谱信号调控效应。红光,尤其是红光655处理的叶面积、鲜质量、干质量显著高于对照57.89%~85.04%;尽管蓝光下全株及叶和根的可溶性蛋白质含量均显著高于对照33.26%~181.23%,可是随着光谱波长的减小,可溶性蛋白质在各器官中的分配更加呈现以叶为主的特征。相比之下,随着光谱波长的增加,可溶性糖在各器官中的分配更加呈现以叶为主的特征。此外,蓝光、红光处理全株以及叶和茎器官的可溶性糖含量显著高于对照97.26%~357.54%;红光下叶中的游离氨基酸含量低于对照52.79%~59.01%,且明显抑制了GOT活性,以及游离氨基酸分配以茎为主和根其次的特征,暗示着红光有利于氨基酸向阴生器官或部位转运分配的倾向。
Based on the differences in the intensity of chlorophyll absorption spectra of higher plants, we analyzed the changes of the chlorophyll absorption spectra with the wavelengths of 442 ± 9 nm, 457 ± 7 nm, 521 ± 14 nm, 627 ± 7 nm and 655 ± 11) nm red light emitting diode (LED) light source was irradiated with lettuce at a dose of 300μmol · m -2 (s -1) for 12 h · d -1 Week, with greenhouse natural light as a control, with a certain energy supply to the lower leaves of lettuce growth and nutrient distribution of spectral signal regulation and control effects. Leaf area, fresh weight and dry weight of red light, especially red light 655, were significantly higher than those of the control 57.89% ~ 85.04%. Although soluble protein content of whole plant and leaves and roots were significantly higher than that of the control 33.26% ~ 181.23 %, But as the spectral wavelength decreases, the distribution of soluble proteins in various organs is more predominantly leaf-based. In contrast, as the spectral wavelength increases, the distribution of soluble sugars in various organs presents more leaf-dominated features. In addition, soluble sugar content in whole plant, leaves and stem organs was significantly higher than that in the control (97.26% -357.54%); the contents of free amino acids in the lower leaves were lower than the control (52.79% -59.01%) and significantly inhibited GOT activity, and the secondary distribution of free amino acids, mainly stems and roots, implied that red light favored the translocation and distribution of amino acids into the perineal organs or organs.