本文评价严重精子减少症将精子移植至成熟卵的卵周间隙,研究了用酸性磷酸盐缓冲液(PBS)做透明带钻孔法以及存在细胞松弛素D时作透明带直接穿刺法。此外,透明带钻孔法也用于曾经人工授精失败而配子正常的妇女。研究对象为不孕夫妇,丈夫精子重度减少(精子数少于每毫升5百万活动精子);或光镜检查配子外表正常。患者均经IVF和配子输卵管移植失败。从7个其丈夫患精子重度减少症的妇女及3个IVF失败者获得47个卵子。取 In this review, severe sperm depletion was assessed in which the sperm were transplanted into the perivitelline space of mature eggs. The zona pellucida direct puncture was performed using a zona pellucida drilling method with acidic phosphate-buffered saline (PBS) and cytochalasin D present. In addition, the zona pellucida method is also used for women who failed to have artificial insemination and had normal gametes. Subjects for infertile couples, her husband severe reduction of sperm (sperm count less than 5 million per ml of active sperm); or light microscopy to check the appearance of normal gametes. Patients had failed IVF and gametubal tubal transplantation. Seventy eggs were obtained from seven women whose husbands had severe sperm hypo-sperm failure and three from an IVF failure. take