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目的评价不同剂量植物雌激素染料木黄酮与钙、维生素D3联合应用预防卵巢切除小鼠骨质疏松的作用。方法 63只平均体重29g CD-1雌性小鼠随机分为7组,包括Sham组、卵巢切除(OVX)组、OVX给药组分为碳酸钙、维生素D3联合染料木黄酮的高剂量组(GH 67mg/kg)、中剂量组(GM 33.5mg/kg)、低剂量组(GL 16.75mg/kg)3组以及单纯染料木黄酮组、雌激素组(E2)。给药6周,测定小鼠骨密度(BMD)、骨矿含量(BMC)、骨生物力学和骨代谢生化指标。结果 GH、GM、GL组对卵巢切除小鼠的子宫有刺激生长作用,其中GL组作用小。GM组明显增加卵巢切除小鼠的BMD、BMC和股骨长度、股骨宽度,GL组明显增加卵巢切除小鼠的BMD(P<0.01)。GL组对卵巢切除小鼠的股骨最大载荷和最大应力升高最显著(P<0.01)。GL组骨碱性磷酸酶(BALP)明显升高、抗酒石酸酸性磷酸酶(TRAP)显著降低(P<0.01)。结论低剂量植物雌激素染料木黄酮与钙、维生素D3联合应用对卵巢切除小鼠的刺激子宫生长作用小。低剂量植物雌激素染料木黄酮与钙、维生素D3联合应用增加BMD,改善骨生物力学参数,促进骨形成和抑制骨吸收,降低了染料木黄酮的用量,并且较雌激素安全。
Objective To evaluate the effect of different doses of phytoestrogen genistein in combination with calcium and vitamin D3 in prevention of osteoporosis in ovariectomized mice. Methods Sixty-three female SD mice with a mean body weight of 29 g were randomly divided into 7 groups: Sham group, ovariectomized (OVX) group, high dose of OVX administration group with calcium carbonate and vitamin D3 plus genistein 67 mg / kg), middle dose group (GM 33.5 mg / kg), low dose group (GL 16.75 mg / kg), simple genistein group and estrogen group (E2) After 6 weeks of administration, the bone mineral density (BMD), bone mineral content (BMC), bone biomechanics and biochemical markers of bone metabolism were measured. Results The GH, GM and GL groups stimulated the growth of the uterus of ovariectomized mice, and the effect of GL was small. In GM group, BMD, BMC, femur length and femur width were significantly increased in ovariectomized mice, while GL group significantly increased BMD in ovariectomized mice (P <0.01). The GL group had the most significant increase in femur maximum load and stress in ovariectomized mice (P <0.01). GL had a significant increase in bone alkaline phosphatase (BALP) and a significant decrease in tartrate-resistant acid phosphatase (TRAP) (P <0.01). Conclusions The combination of low dose phytoestrogen genistein with calcium and vitamin D3 has little effect on uterine growth in ovariectomized mice. Low doses of phytoestrogen genistein combined with calcium and vitamin D3 increase BMD, improve bone biomechanical parameters, promote bone formation and inhibit bone resorption, reduce the dosage of genistein, and are safer than estrogen.