论文部分内容阅读
目的 探讨不同的活检方法、活检时机和活检细胞数对胚胎体外发育能力的影响。方法 选取体外受精 胚胎移植剩余的形态学分级为Ⅰ级的胚胎 1 54个 ,对其中第 1阶段的 66个胚胎分别行化学法 (2 6个 )、机械法 (2 0个 )取出 1个卵裂球 ,并设对照 (2 0个 ) ;对第 2阶段的 88个胚胎分为用化学法取出 2个卵裂球 (44个 )和对照 (44个 )。观察记录活检时胚胎细胞数、活检时间、活检后卵裂球是否退化、活检后胚胎体外发育情况及囊胚总细胞数。结果 (1 )化学法的活检时间为 (2 31±2 0 )s,明显短于机械法的 (2 62± 2 3)s(P <0 0 1 ) ;而囊胚形成率为 65 % ,高于机械法的 35 % (P<0 0 5)。(2 ) 6 细胞胚胎的囊胚总细胞数为 (44± 4)个 ,低于 7～ 8 细胞胚胎的 (49± 5)个和≥ 9 细胞胚胎的 (50± 6)个 (P <0 0 5) ;细胞融合的≥ 9 细胞胚胎活检后不仅囊胚形成率 (2 0 % )低于对照 (67% ,P<0 0 5) ,且活检后卵裂球退化率 (50 % )高于无细胞融合的胚胎 (1 7% ,P <0 0 5)。 (3)取出 1个或 2个卵裂球胚胎的囊胚形成率和囊胚总细胞数与对照比较 ,差异均无显著性 (P >0 0 5) ,而 6 细胞胚胎取出 2个卵裂球后囊胚形成率 (1 /8)低于对照 (5/8,P <0 0 5)。结论 化学法活检比机械法更为快速、安全 ;合适的活检时
Objective To investigate the different biopsy methods, biopsy timing and the number of biopsy cells on embryonic development in vitro. Methods One hundred and seventy-four embryos of stage Ⅰ embryos were obtained by in vitro fertilization embryo transfer. Sixty-six embryos of the first stage were subjected to chemical method (26) and mechanical method (20), respectively. The blastomeres were divided into two groups (control group). The 88 embryos in the second stage were divided into two blastomeres (44) and the control (44) by chemical methods. Observe and record the number of embryo cells during biopsy, the time of biopsy, the degenerative of blastomere after biopsy, the in vitro development of embryos after biopsy and the total number of blastocysts. Results The biopsy time of (1) chemical method was (21 31 ± 20) s, which was significantly shorter than that of mechanical method (22 62 ± 23 s) (P <0.01), while the blastocyst formation rate was 65% 35% higher than the mechanical method (P <0 05). (2) The number of total blastocysts of 6-cell embryos was (44 ± 4), (49 ± 5) less than 7-8 embryos and (50 ± 6) The rate of blastocyst formation (20%) was lower than that of control (67%, P <0 05) after biopsy of ≥9 cell embryos and the degenerative rate of blastomere (50%) after biopsy On embryos without cell fusion (17%, P <0 05). (3) The blastocyst formation rate and total number of blastocysts in one or two blastomere embryos were not significantly different from those in control (P> 0.05), while the number of blastocysts in 6-cell embryos was 2 The rate of posterior blastocyst formation (1/8) was lower than that of the control (5/8, P <0 05). Conclusions Chemical biopsy is faster and more secure than mechanical methods; when biopsy is appropriate