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从湖北省32种植物上取受根结线虫(Meloidogyne spp.)危害的根和根围土壤64份样品,采用3种方法分离,经过筛选测定,获寄生能力较强的真菌2株,室内凹玻片和琼脂平板法测定结果表明,对卵的校正寄生率分别为78.04%和70.02%;室外盆栽测定的寄生率分别为72.37%和65.65%,供试卵有效孵化率为5.02%和8.32%,对照卵为100%。4%土重的棉籽壳真菌制剂能有效控制根结线虫第一代卵量,有效用量的10倍菌剂盆栽测定对植物均没有不良影响。因此该菌株是有希望的防治植物根结线虫病的真菌。
Sixty-four samples of roots and rhizosphere soils contaminated by Meloidogyne spp. Were collected from 32 species of plants in Hubei Province and separated by 3 methods. After screening and screening, 2 strains of parasitic fungi were found, The results of slide and agar plate method showed that the corrected parasitism rates of eggs were 78.04% and 70.02%, respectively. The parasitism rates of outdoor potted plants were 72.37% and 65.65%, respectively. The effective hatching rates of test eggs were 5.02% and 8.32% , Control egg is 100%. 4% soil weight of cottonseed fungus preparations can effectively control the first generation eggplant root knot nematode amount of 10 times the effective dose of potted plant determination without adverse effects on plants. This strain is therefore a promising fungus for controlling root knot nematodes.