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目的研究紫杉醇分子印迹聚合物在固相萃取中的应用。方法采用分子印迹沉淀合成技术,以紫杉醇为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸为交联剂、偶氮二异丁腈为引发剂,在氯仿与甲苯混合液中合成紫杉醇分子印迹聚合物,并以此为填料进行固相萃取,分离纯化紫杉醇。印迹聚合物对紫杉醇的吸附选择性优化聚合反应条件,通过平衡结合法和Scatchard模型评价聚合物吸附特点,再用固相萃取技术对清洗洗脱方法进行优化,采用实际提取浸膏考察固相萃取柱的可用性。结果甲基丙烯酸和紫杉醇的摩尔比为4∶1,聚合温度为60℃时,分子印迹聚合物(molecularly imprinted polymers,MIPs)对紫杉醇呈现高特异性吸附;印迹吸附符合Scatchard模型,计算MIPs对紫杉醇的最大表观结合点数为3.66 mg·g~(-1),K_d为6.627。结论分子印迹聚合物可以为紫杉醇的分离纯化提供新型选择性富集材料。
Objective To study the application of paclitaxel molecularly imprinted polymer in solid phase extraction. Methods The molecularly imprinted precipitation synthesis technique was used. Paclitaxel was used as the template molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the crosslinking agent, azobisisobutyronitrile as the initiator, and the mixture of chloroform and toluene In paclitaxel molecularly imprinted polymer, and as a filler for solid phase extraction, separation and purification of paclitaxel. The adsorption selectivity of the imprinted polymer to paclitaxel was optimized. The adsorption characteristics of the polymer were evaluated by the equilibrium binding method and the Scatchard model. The solid phase extraction (SPE) was used to optimize the elution and elution methods. Column availability. Results The molar ratio of methacrylic acid to paclitaxel was 4:1, and molecularly imprinted polymers (MIPs) showed high specific adsorption to paclitaxel when the molar ratio of methacrylic acid to paclitaxel was 4:1. According to the Scatchard model, the adsorption of MIPs to paclitaxel The maximum number of apparent binding sites was 3.66 mg · g -1, K_d was 6.627. Conclusion The molecularly imprinted polymer can provide a novel and selective enrichment material for the purification of paclitaxel.