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目的探讨慢性前列腺炎(CP)患者前列腺液(EPS)中活性氧(ROS)表达变化与CP类型、EPS中WBC计数的相关关系。方法按照NIH分类标准,根据EPS常规检查,二杯法细菌培养,诊断CP患者87例,其中Ⅱ型10例、ⅢA型30例、ⅢB型30例、Ⅳ型17例,26例健康志愿者作正常对照组。显色法检测各组ROS值并进行相关分析。结果各组ROS数据均符合正态分布, P>0．1。其中Ⅱ型组ROS均值(233．1±56．1)U／ml,ⅢA型组(180．5±70．7)U／ml,ⅢB型组(156．8±38．9)U／ml,Ⅳ型组(124．9 4-34．1)U／ml,正常对照组(87．9±26．3)U／ml。CP患者EPS中ROS值明显高于对照组。Ⅱ型组、ⅢA型组、ⅢB型组、Ⅳ型组、正常对照组的ROS水平呈现从高到低的变化规律,其中Ⅱ型与Ⅲ型、ⅢA型与Ⅳ型之间差异有统计学意义(P<0．01),ⅢB型与Ⅳ型、Ⅳ型与正常对照组之间差异有统计学意义(P<0．05)。ⅢA型与ⅢB型组间差异无统计学意义(P> 0．05)。CP患者EPS中WBC计数与ROS水平呈正相关性(r=0．275,P=0．01),87例患者按EPS中WBC数分组:0—9个／HP组30例,ROS为(153．7±35．9)U／ml;10～19个／HP组16例,ROS为(153．3±44．8)U／ml;20～49个／HP组24例,ROS为(163．7±57．1)U／ml;≥50个／HP组17例,ROS为(205．3±929)U／ml。各组间ROS值差异有统计学意义(F=3．297,P<0．05)。卵磷脂小体含量与ROS水平呈负相关性(r=-0．31,P<0．01),87例患者按EPS中卵磷脂小体含量分组:(+)组22例, ROS值为(187．8±76．4)U／ml;(++)组32例,ROS值为(173．6±53．7)U／ml;(+++)组24例, ROS值为(160．0±49．7)U／ml;(++++)组9例,ROS值为(117．0±43．5)U／ml。各组间ROS值差异有统计学意义(F=3．363,P<0．05)。结论CP患者EPS中ROS表达增高,并参与前列腺炎的发生发展,可作为CP临床诊断和严重程度定量参考依据之一。
Objective To investigate the relationship between reactive oxygen species (ROS) expression in prostatic fluid (EPS) and type of CP in patients with chronic prostatitis (CP) and count of WBC in EPS. Methods According to the NIH classification criteria, 87 patients with CP were diagnosed according to the routine examination of EPS and bacterial culture by two cup method, including 10 cases of type Ⅱ, 30 cases of type ⅢA, 30 cases of type ⅢB, 17 cases of type Ⅳ and 26 cases of healthy volunteers Normal control group. The colorimetric method was used to detect the ROS value in each group and the correlation analysis was made. Results The ROS data of all the groups were in accordance with the normal distribution, P> 0.1. The mean ROS level of type II group (233.1 ± 56.1) U / ml, type ⅢA group (180.5 ± 70.7) U / ml, type ⅢB group (156.8 ± 38.9) U / ml , Type Ⅳ (124.9 4-34.1) U / ml, normal control group (87.9 ± 26.3) U / ml. CP patients with ROS levels were significantly higher than the control group. The levels of ROS in type Ⅱ, type ⅢA, type ⅢB, type Ⅳ and normal control groups showed a pattern of change from high to low, of which the difference between type Ⅱ and type Ⅲ, type ⅢA and type Ⅳ was statistically significant (P <0.01). There was a significant difference between type ⅢB and type Ⅳ, type Ⅳ and normal control group (P <0.05). There was no significant difference between ⅢA and ⅢB (P> 0.05). There was a positive correlation between WBC count and ROS level in CP patients (r = 0.275, P = 0.01) .87 patients were grouped according to the number of WBC in EPS group: 0-9 / HP group, and ROS was 153 .7 ± 35.9) U / ml; 16 cases in 10 ~ 19 / HP group, 153.3 ± 44.8 U / ml in ROS; 24 cases in 20 ~ 49 / .7 ± 57.1) U / ml; ≥50 / HP group, 17 cases of ROS (205.3 ± 929) U / ml. There was a significant difference in ROS values among the three groups (F = 3.297, P <0.05). The content of lecithin was negatively correlated with the level of ROS (r = -0.31, P <0.01). According to the lecithin content in EPS group, 87 patients were divided into (+) group and 22 (++) group (32 cases), ROS value was (173.6 +/- 53.7) U / ml; (+++) 160.0 ± 49.7) U / ml; (++++) group, 9 cases, ROS value was (117.0 ± 43.5) U / ml. There was a significant difference in ROS values among the three groups (F = 3.363, P <0.05). Conclusion The expression of ROS in EPS is increased in CP patients and is involved in the occurrence and development of prostatitis. It may be used as a quantitative basis for the clinical diagnosis and severity of CP.