观测２４具防腐的成人尸体Ｌ＿５～Ｓ＿１椎间盘的断面标本和解剖８具成人尸体Ｌ＿５～Ｓ＿１椎间盘的毗邻结构，证实Ｌ＿５～Ｓ＿１椎间盘存在三个可供穿刺的部位：前区、侧区、后外侧区。穿刺Ｌ＿５～Ｓ＿１椎间盘当首选后外侧区。该区位于椎关节实和Ｌ＿５脊神经前支之间，仅见于椎间盘的中、下部断面，间隙宽度分别为６．７ｍｍ和９．９ｍｍ。穿刺针从髂后上棘外上方３．５～４．０ｃｍ处进针，向第５腰椎横突下方的骨孔插入，一般都能到达椎间盘中心。 Observed 24 anticorrosive adult cadaveric L_5 ~ S_1 intervertebral disc sections and anatomized 8 adult cadaveric L_5 ~ S_1 intervertebral disc adjacent structures, confirmed L_5 ~ S_1 intervertebral disc there are three sites for puncture: anterior, lateral, posterolateral Area. Puncture L_5 ~ S_1 disc when the preferred posterolateral region. The area is located between the spondylosis and L_5 anterior branch of the spinal nerve, found only in the middle and lower disc section, the gap width of 6.7mm and 9.9mm. Puncture needle from the posterior superior iliac spine outside the 3.5 ~ 4.0cm at the needle, the fifth lumbar transverse process below the bone into the hole, the general can reach the center of the disc.