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【目的】明确转hpa1Xoo棉花抗虫性及其作用机制。【方法】采用生测法和芯片技术分析了转hpa1Xoo棉T-34的抗虫性和harpinXoo表达对棉铃虫全基因组转录谱的影响。【结果】饲喂T-34棉叶的棉铃虫发育明显迟缓,棉铃虫死亡率为90.95%,而饲喂其出发品种(Z35)棉叶的棉铃虫死亡率为12.20%。利用和棉铃虫同属鳞翅目的家蚕的23KOligo探针,以分别饲喂T-34和Z35叶片的棉铃虫RNA为模板,制作了棉铃虫Oligo表达谱芯片,获得了24280个EST数据,从中检测到了2927个基因。在T-34和Z35上饲喂的棉铃虫有872个差异表达基因,其中上调基因328个(ratio>2.0),下调基因544个(ratio<0.5)。872个差异表达基因主要涉及13个生物功能、96个代谢通路。【结论】转hpa1Xoo棉花对棉铃虫的抗性涉及棉铃虫蛋白酶分解和合成、ATP合成等多个生物代谢途径的改变。
【Objective】 The purpose of this study was to determine the insect resistance and its mechanism of transgenic cotton hpa1Xoo. 【Method】 The effects of insect resistance of transgenic hpa1Xoo cotton T-34 and harpinXoo expression on the genome-wide transcriptional profile of cotton bollworm (Helicoverpa armigera) were analyzed by using bioassay and microarray technology. 【Result】 The results showed that the development of cotton bollworm (T-34) was obviously delayed. The mortality of cotton bollworm was 90.95%, while the death rate of cotton bollworm (Z35) was 12.20%. Using Helicoverpa armigera, a 23KOligo probe of silkworm belonging to Lepidoptera, cotton bollworm RNA of T-34 and Z35 was used as a template to produce cotton bollworm Oligo cDNA microarray. 24280 ESTs were obtained, 2927 genes. There are 872 differentially expressed genes in the cotton bollworm fed on T-34 and Z35, including 328 up-regulated genes (ratio> 2.0) and 544 down-regulated genes (ratio <0.5). 872 differentially expressed genes mainly involved 13 biological functions and 96 metabolic pathways. 【Conclusion】 The resistance of transgenic hpa1Xoo cotton to cotton bollworm involves the changes of several biological metabolic pathways, such as proteolytic decomposition and synthesis of cotton bollworm, and ATP synthesis.