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问:用HPLC进行分析时保留时间有时发生漂移,有时发生快速变化,原因何在?如何解决? 答:关于漂移问题: 1.温度控制不好,解决方法是采用恒温装置,保持柱温恒定 2.流动相发生变化,解决办法是防止流动相发生蒸发、反应等 3.柱子未平衡好,需对柱子进行更长时间的平衡 关于快速变化问题: 1.流速发生变化,解决办法是重新设定流速,使之保持稳定
Q: When using HPLC for analysis, retention time sometimes drifts and sometimes rapid changes occur. What are the causes? How to solve? A: Regarding the drift problem: 1. The temperature control is not good. The solution is to use constant temperature device to keep the column temperature constant. The mobile phase changes, the solution is to prevent the mobile phase evaporation, reaction, etc. 3. The column is not balanced, the need for a longer time to balance the column About rapid changes: 1. Flow rate changes, the solution is to reset the flow rate , Make it stable