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目的:探讨姜黄素对急性坏死性胰腺炎(ANP)大鼠NF-κB活性的调节作用。方法:将SD大鼠随机分为胰腺炎组(ANP组)和姜黄素组(Cur组),通过分次腹腔注射10%L-精氨酸建立大鼠ANP模型,Cur组在造模前20天开始每天给予姜黄素灌胃。两组分别在造模后3h、12h、24h和36h处死大鼠,观察胰腺病理改变、NF-κB活性和血清淀粉酶、MDA、TNF-α、IL-1β的变化。结果:Cur组3h、12h、24h和36h时间点的与ANP组对应时间点比较,胰腺病理改变P=0.049,0.016,0.003,0.001;胰腺组织NF-κB活性P=0.001,0.001,0.006,0.001;淀粉酶P=0.043,0.001,0.002,0.001;丙二醛P=0.026,0.048,0.025,0.000;IL-1βP=0.016,0.016,0.002,0.006;TNF-αP=0.039,0.018,0.005,0.002。结论:姜黄素可能通过抑制NF-κB活性来下调细胞因子水平,并减轻胰腺损伤。
Objective: To investigate the effect of curcumin on the activity of NF-κB in rats with acute necrotizing pancreatitis (ANP). METHODS: Sprague-Dawley rats were randomly divided into pancreatitis group (ANP group) and curcumin group (Cur group). Rat ANP model was established by intraperitoneal injection of 10% L-arginine. The Cur group was before modeling. Curcumin was given daily by gavage. The rats were killed at 3h, 12h, 24h and 36h after modeling. The pathological changes of the pancreas, NF-κB activity, and the changes of serum amylase, MDA, TNF-α and IL-1β were observed. RESULTS: Compared with the ANP group at 3h, 12h, 24h and 36h time points in the Cur group, the pathological changes of the pancreas were P=0.049, 0.016, 0.003, 0.001; the activity of NF-κB in the pancreas was P=0.001, 0.001, 0.006, 0.001 Amylase P=0.043, 0.001, 0.002, 0.001; Malondialdehyde P=0.026, 0.048, 0.025, 0.000; IL-1βP=0.016, 0.016, 0.002, 0.006; TNF-αP=0.039, 0.018, 0.005, 0.002. Conclusion: Curcumin may down-regulate cytokines and inhibit pancreatic injury by inhibiting NF-κB activity.