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目的:探讨心衰宁颗粒对慢性心力衰竭大鼠心肌转化生长因子β1(TGFβ1)表达的影响。方法:将SD大鼠随机分为正常组、模型组、卡托普利组、心衰宁大、中、小剂量组,除正常组外,其余组采用阿霉素腹腔注射制作慢性心力衰竭大鼠模型,正常组注射等量生理盐水,每周1次,造模6周结束后,分别给予药物干预,正常组及模型组给予生理盐水,干预3周。干预结束后,超声心动图检测心功能,大鼠血清学检测pro BNP、TGFβ1水平;qRT-PCR、Western blot检测检测TGFβ1基因及蛋白表达水平。结果:与正常组比较,模型组左心室舒张末期内径(LVEDD)和左心室收缩末期内径(LVESD)明显增加(P<0.01),左室射血分数(LVEF)降低(P<0.01),血清TGF-β1、pro BNP明显升高(P<0.01),TGF-β1基因及蛋白表达较正常组明显升高(P<0.01);与模型组比较,心衰宁颗粒中、大剂量组能降低LVEDD、LVESD(P<0.05),提高慢性心衰大鼠LVEF(P<0.01),降低血清TGF-β1、pro BNP水平(P<0.01),降低TGF-β1基因及蛋白表达水平(P<0.05)。结论:心衰宁颗粒具有改善心功能,干预心肌重塑、抗心肌纤维化作用,其机制可能与抑制TGF-β1水平有关。
Objective: To investigate the effect of xinhuning granule on the expression of transforming growth factor β1 (TGFβ1) in rats with chronic heart failure. Methods: The SD rats were randomly divided into normal group, model group, captopril group, large, medium and small dose of heart failure group, except the normal group, the other group were injected with doxorubicin for chronic heart failure The rats in the normal group were injected with the same amount of saline once a week for 6 weeks. After the 6 weeks, the animals were given drug intervention. The normal group and the model group were given physiological saline for 3 weeks. After the intervention, heart function was detected by echocardiography, and serum proBNP and TGFβ1 levels were detected by qRT-PCR and Western blot. TGFβ1 gene and protein expression levels were detected by qRT-PCR. Results: Compared with the normal group, the left ventricular end-diastolic dimension (LVEDD) and left ventricular end-systolic dimension (LVESD) in the model group increased significantly (P <0.01), the left ventricular ejection fraction (LVEF) decreased TGF-β1 and pro BNP were significantly increased (P <0.01), TGF-β1 gene and protein expression was significantly higher than the normal group (P <0.01); compared with the model group, LVEDD and LVESD (P <0.05), and increased the LVEF (P <0.01), decreased the levels of serum TGF-β1 and pro BNP (P <0.01) and decreased the expression of TGF-β1 in rats with chronic heart failure ). Conclusion: XINHONGNING granule can improve cardiac function, interfere myocardial remodeling and anti-myocardial fibrosis, and its mechanism may be related to inhibiting the level of TGF-β1.