论文部分内容阅读
目的探讨姜黄素对人脑胶质瘤U251细胞侵袭与迁移的抑制作用。方法采用四甲基偶氮唑蓝法筛选姜黄素实验浓度;采用细胞划痕实验观察姜黄素作用0、6、12和24 h后胶质瘤U251细胞的迁移情况,并且测量24 h后迁移的距离;采用Transwell实验观察穿膜细胞数,以判断细胞的侵袭能力;采用Western blotting实验检测胶质瘤U251细胞中侵袭相关蛋白基质金属蛋白酶2(matrix metalloproteinase-2,MMP2)和基质金属蛋白酶9的表达。结果四甲基偶氮唑蓝法筛选的姜黄素浓度为10和20μmol·L-1;与空白对照组相比,在姜黄素作用24 h后,胶质瘤U251细胞迁移的距离和穿膜细胞数皆明显减少,且与药物浓度呈正相关(P<0.01);与空白对照组相比,姜黄素组胶质瘤U251细胞内基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达明显下降(P<0.01),在姜黄素10和20μmol·L-1组基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达差异明显(P<0.05)。结论姜黄素能够抑制人脑胶质瘤U251细胞的迁移与侵袭,可能是通过下调细胞内基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达引起的。
Objective To investigate the inhibitory effect of curcumin on the invasion and migration of human glioma U251 cells. Methods The concentration of curcumin was screened by MTT method. The migration of glioma cells U251 after curcumin treatment at 0, 6, 12 and 24 h was observed by cell scratch assay. The migration of The number of transmembrane cells was detected by Transwell assay to determine the invasion ability of cells. Western blotting was used to detect the expression of invasion-related protein matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase 9 in glioma U251 cells expression. Results The concentration of curcumin screened by MTT assay was 10 and 20 μmol·L-1, respectively. Compared with the blank control group, the migration distance of glioma U251 cells and the number of transmembrane cells (P <0.01). Compared with the blank control group, the expression of MMP-2 and MMP-9 in glioma U251 cells of curcumin group was significantly decreased (P < 0.01). The expressions of MMP-2 and MMP-9 in curcumin 10 and 20 μmol·L-1 groups were significantly different (P <0.05). Conclusion Curcumin can inhibit the migration and invasion of human glioma U251 cells by down-regulating the expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 protein.