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本实验利用转基因小鼠,研究了β胰岛细胞上谷氨酸脱羧酶(ＧＡＤ)的表达与非肥胖糖尿病(ＮＯＤ)小鼠糖尿病发病的确切关系。将两种不同变异型的小鼠ＧＡＤｃＤＮＡ(ｒＧＡＤ65和ｒＧＡＤ67)的反义基因转移到ＮＯＤ小鼠的胚系细胞上,然后将这些干细胞植入雌性ＮＯＤ鼠,经传代得到
In this experiment, the exact relationship between the expression of glutamic acid decarboxylase (GAD) in pancreatic islet cells and the onset of diabetes in non-obese diabetic (NOD) mice was studied using transgenic mice. Antisense genes of two different variant mouse GAD cDNAs (rGAD65 and rGAD67) were transferred to germ line cells of NOD mice and these stem cells were then seeded into female NOD mice and passaged to give