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以粳粳交优质高产的东农425/长白10号F2:3代180个家系为作图群体,在25 mmol·L-1NaHCO3溶液碱环境胁迫下,对水稻进行耐碱性鉴定,并依据SSR标记构建的分子连锁图谱,对水稻幼苗前期的根数、根长和叶绿素含量及其相对碱害率进行数量性状基因座(QTLs)的检测。结果表明,在F2 3株系群中,水稻的根数、根长和叶绿素含量及其相对碱害率均呈单峰连续的正态分布。共检测到在碱胁迫下与水稻幼苗前期根数、根长、叶绿素含量及其相对碱害率相关的16个QTLs,分别位于第1、3、6、7、8、9、10、11和12染色体上。其中与根数相关的QTL 3个;与根数相对碱害率相关的QTL 1个;与根长相关的QTL 2个,与根长相对碱害率相关的QTL 2个;与叶绿素含量相关的QTL 2个;与叶绿素含量相对碱害率相关的QTL 6个。研究结果可作为水稻耐碱性QTL精细定位和分子辅助选择育种的理论基础和科学依据。
A total of 180 F2: 3 generations of Dongnong 425 and Changbai 10 with good quality and high yielding japonica-japonica hybrid rice were selected as the mapping population. The alkaline resistance of rice was evaluated under 25 mmol·L-1 NaHCO3 solution under alkali stress. The SSR The constructed molecular linkage map was used to detect quantitative trait loci (QTLs) of root number, root length, chlorophyll content and relative alkali damage rate in early rice seedling stage. The results showed that the root number, root length, chlorophyll content and relative alkali damage rate of rice in F2 3 were all single peak continuous normal distribution. A total of 16 QTLs related to root number, root length, chlorophyll content and their relative alkali damage rate were detected under alkaline stress at the first, third, sixth, seventh, eighth, ninth, tenth, eleventh and 12 chromosomes. Among them, there were 3 QTLs related to root number, 1 QTL related to root relative rate, 2 QTLs related to root length and 2 QTLs related to root length. 2 QTLs and 6 QTLs related to chlorophyll content. The results could be used as the theoretical basis and scientific basis for the fine mapping and molecular-assisted selection breeding of Alkali-resistant QTL in rice.