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以邻苯二酚溶液为底物,采用分光光度法在410nm处测定小麦籽粒多酚氧化酶活性大小。并研究反应温度、底物浓度和酶液浓度等因素对其活性测定的影响。结果表明:以上测定指标对酶活性测定的影响非常明显。
Using catechol solution as substrate, the polyphenol oxidase activity of wheat grain was determined by spectrophotometry at 410 nm. The effect of reaction temperature, substrate concentration and enzyme concentration on the determination of its activity was also studied. The results show that: the above determination of the impact of enzyme activity determination is very obvious.