采用牛津杯法,研究了鹿蹄草素对链格孢病菌的抑制作用。通过单因子-正交实验方法,分别考察了不同浓度、不同溶剂和不同pH3个因素对鹿蹄草素抑制链格孢病菌效果的影响。实验结果表明,最佳抑菌条件为:鹿蹄草素浓度:5mg/mL,pH:7~8;溶剂:60%乙醇。 Oxford cup method was used to study the inhibitory effect of P. frugiperda on Alternaria alternata. The single factor-orthogonal experiment method was used to investigate the effects of different concentrations, different solvents and different pH on the inhibitory effect of P. frugiperda on Alternaria alternata. The experimental results showed that the optimal antibacterial conditions were as follows: Fruclostatin concentration: 5 mg/mL, pH: 7~8; solvent: 60% ethanol.