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A field study was undertaken to ascertain the effects of elicitors viz.benzothiadiazole(BTH)and salicylic acid(SA)on defense related enzymes viz.peroxidase,phenylalanine ammonia lyase,superoxide dismutase,chitinase andβ-1,3-glucanase,and phenols in rice(Pusa Basmati I)plants.First foliar spray of BTH(50 mg/kg)and SA(50 mg/kg)was done at the maximum tillering stage and inoculation with Rhizoctonia solani was carried 24 h after elicitor treatment.Elicitors were further sprayed at every growth stage.Time course analysis showed peak accumulation of defense related enzymes and phenols in the rice leaves treated with BTH and SA,and accumulation was the highest at the flowering stage.Higher enzymatic activity was observed in elicitor treated plants inoculated with R.solani.Compared to the untreated control plants,application of elicitors before R.solani inoculation significantly elicited the defense related enzymes and phenols.Moreover,application of elicitors had a positive effect on yield and disease reduction.It is suggested that pretreatment of rice leaves with BTH and SA could be used to enhance the level of protection against sheath blight and to improve rice yield in the fields.
A field study was undertaken to ascertain the effects of elicitors viz.benzothiadiazole (BTH) and salicylic acid (SA) on defense related enzymes viz.peroxidase, phenylalanine ammonia lyase, superoxide dismutase, chitinase and β- 1,3-glucanase, and phenols in Rice (Pusa Basmati I) plants. First foliar spray of BTH (50 mg / kg) and SA (50 mg / kg) was done at the maximum tillering stage and inoculation with Rhizoctonia solani was carried 24 h after elicitor treatment. sprayed at every growth stage .ime course analysis showed peak accumulation of defense related enzymes and phenols in the rice leaves treated with BTH and SA, and accumulation was the highest at the flowering stage .Higher enzymatic activity was observed in elicitor treated plants inoculated with R . solani .Compared to the untreated control plants, application of elicitors before R.solani inoculation significantly elicited the defense related enzymes and phenols. Moreover, application of elicitors had a positive effect on yield and disease reduction. It is suggested that pretreatment of rice leaves with BTH and SA could be used to enhance the level of protection against sheath blight and to improve rice yield in the fields.