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以石蒜鳞茎为外植体,研究了不同激素组合、鳞茎不同部位和不同生长时期等因素对石蒜愈伤组织诱导的影响及其继代培养。结果表明:MS+2,4-D 1 mg·L~(-1)+6-BA 1 mg·L~(-1)激素组合能较好的诱导出石蒜愈伤组织,诱导率达61.13%;外植体的选择是石蒜愈伤组织诱导的关键因素,内层鳞茎诱导愈伤组织的效果最好;在一个生长周期中,9、10月的鳞茎作为外植体诱导愈伤组织最佳;MS+2,4-D 0.5 mg·L~(-1)+KT 0.5 mg·L~(-1)是愈伤组织较好的继代培养基,继代周期为24～27 d。
The effects of different combinations of hormones, different parts of the bulbs and different growth stages on the callus induction of Lycoris radiata and its subculture were studied by using Lycoris radiate bulbs as explants. The results showed that MS + 2, 4-D 1 mg · L -1 and 6-BA 1 mg · L -1 could induce the callus of Lycoris well, the induction rate was 61.13% . The explant selection was the key factor for the callus induction of Lycoris radiata and the callus induction by the inner bulb was the best. In one growth cycle, the bulbs in September and October as the explants, MS + 2,4-D 0.5 mg · L -1 + KT 0.5 mg · L -1 was the better subculture medium for callus, with a subculture period of 24-27 days.