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RAPD(random amplified polymorphic DNA,随机扩增多态性DNA)技术自1990年由Williams及Welsh等人几乎同时发展起来后,该技术在中药鉴定方面得到了较广泛的应用。其基本原理是采用随机序列的较短的单个随机引物(10碱基寡核苷酸),以提取的药材总DNA为模板,引物与
RAPD (random amplified polymorphic DNA) technology has been developed almost simultaneously by Williams and Welsh et al. in 1990. The technology has been widely used in the identification of traditional Chinese medicines. The basic principle is to use a random sequence of shorter single random primers (10 base oligonucleotides) to extract the total DNA of the drug as a template, primers and