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目的:由于Ubc9在肿瘤的发生于恶化中发挥巨大作用,本研究目的是对泛素结合酶(Ubc9)mRNA和Ubc9蛋白在非小细胞肺癌组织中表达进行检测,评估Ubc9在肺癌预后中的指导意义。方法:通过荧光定量PCR、免疫组织化学法、Western-blot检测100例非小细胞肺癌病人中Ubc9 mRNA、蛋白水平的表达进一步研究Ubc9的表达与肺癌临床特征的关系。结果:实验结果显示在肺癌细胞中Ubc9阳性表达显示为黄棕色颗粒,与癌旁组织比较,Ubc9 mRNA、蛋白在肺癌组织中高表达,并且与肺癌的临床分型(分期、淋巴结转移、吸烟、分化)有关。Ubc9 mRNA、蛋白在肺癌中的表达癌组织高于癌旁、有淋巴结转移的高于无转移、吸烟高于不吸烟者、低分化组织高于高分化组织。结论:由此可见Ubc9 mRNA、蛋白水平的高表达可能对肺癌临床特征的评估,以及预测术后生存率都有重要指导意义。
OBJECTIVE: As Ubc9 plays an important role in the progression of tumor progression, the aim of this study was to examine the expression of Ubc9 mRNA and Ubc9 protein in non-small cell lung cancer (NSCLC) and assess the role of Ubc9 in the prognosis of lung cancer significance. Methods: The expression of Ubc9 mRNA and protein in 100 patients with non-small cell lung cancer was detected by real-time PCR, immunohistochemistry and Western-blot. The relationship between the expression of Ubc9 and the clinical features of lung cancer was further studied. Results: The results showed that the expression of Ubc9 in lung cancer cells was yellow-brown granules. Ubc9 mRNA and protein were overexpressed in lung cancer tissues compared with adjacent tissues, and were correlated with clinical stage (lymph node metastasis, smoking, differentiation )related. The expression of Ubc9 mRNA and protein in lung cancer was higher than that in cancer, lymph node metastasis was higher than non-metastasis, smoking was higher than non-smoker, and poorly differentiated tissue was higher than well differentiated tissue. Conclusions: Thus, the high expression of Ubc9 mRNA and protein may play an important guiding role in assessing the clinical features of lung cancer and predicting the postoperative survival rate.