目的　研究 R2 L 1细胞的凋亡以及神经营养因子对凋亡的影响。方法　形态学观察、细胞活性测定和流式细胞仪分析。结果　 R2 L 1细胞去血清培养 12小时即发生凋亡 ,2 4小时后大部分细胞凋亡 ,48小时后细胞基本上都死亡。结论　 NGF、 NT- 3和抗 p75 NTR抗体能抑制 R2 L 1细胞在去血清培养后发生的凋亡 ,而 BDNF却无此抑制功能 Objective To investigate the apoptosis of R2 L 1 cells and the effect of neurotrophic factor on apoptosis. Methods Morphological observation, cell viability assay and flow cytometry analysis. As a result, R2 L 1 cells were cultured in serum for 12 hours to induce apoptosis. Most of the cells died after 24 hours and almost all died after 48 hours. Conclusion NGF, NT-3 and anti-p75 NTR antibodies can inhibit the apoptosis of R2 L-1 cells after de-serum culture, while BDNF does not inhibit this function