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目的探讨褪黑素(MLT)在体内外对小鼠前胃癌(MFC)细胞的增殖抑制与凋亡诱导作用。方法体内实验:建立荷胃癌小鼠模型,随机分为5组:A.正常对照组;B.荷瘤空白对照组:每日注射生理盐水100 mg/kg;C.小剂量褪黑素组:荷瘤成功后(接种第6天起)每日腹腔注射MLT 25 mg/kg;D.中剂量MLT组:每日腹腔注射MLT 50 mg/kg;E.大剂量MLT组:每日腹腔注射MLT 100 mg/kg。褪黑素连续注射1周后完整剥离小鼠瘤体并测量其重量体积。体外实验:建立不同浓度褪黑素对胃癌细胞干预模型,运用荧光双标染色,流式细胞术、CCK-8等方法检测褪黑素对小鼠前胃癌细胞增殖抑制和凋亡诱导作用。结果与荷瘤空白对照组相比,褪黑素体内明显下调瘤体的重量体积;与空白对照组相比,褪黑素体外明显抑制胃癌细胞增殖活性,细胞凋亡率增加,并使细胞周期阻滞在G2/M期,并呈剂量依赖性。结论褪黑素在体内外均能抑制小鼠前胃癌细胞的增殖活性,并呈时间-剂量依赖性,该作用与促细胞凋亡和细胞周期G2/M期阻滞有关。
Objective To investigate the inhibitory effect of melatonin (MLT) on the proliferation and apoptosis of mouse precancerous mouse (MFC) cells in vitro and in vivo. Methods In vivo experiments: A gastric cancer-bearing mice model was established and randomly divided into 5 groups: A. normal control group; B. tumor-bearing blank control group: daily saline injection 100 mg / kg; C. low-dose melatonin group: After successful tumor inoculation (from the sixth day of inoculation), daily intraperitoneal injection of MLT 25 mg / kg; D. medium dose MLT group: daily intraperitoneal injection of MLT 50 mg / kg; E. high dose MLT group: daily intraperitoneal injection of MLT 100 mg / kg. After one week of continuous injection of melatonin, the mouse tumor was completely stripped and its weight and volume measured. In vitro experiments: To establish a model of gastric cancer cells intervened by different concentrations of melatonin. The proliferation and apoptosis-inducing effects of melatonin on mouse gastric cancer cells were detected by fluorescent double-stained staining, flow cytometry and CCK-8. Results Compared with the blank control group, melatonin significantly reduced the weight and volume of the tumor in vivo. Compared with the blank control group, melatonin significantly inhibited the proliferation activity of gastric cancer cells in vitro and the apoptosis rate increased, Blocked in G2 / M phase and in a dose-dependent manner. Conclusion Melatonin can inhibit the proliferation of mouse gastric cancer cells in vitro and in vivo in a time-and dose-dependent manner, which is related to the promotion of apoptosis and cell cycle G2 / M arrest.