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目的观察大黄虫丸对梗阻性肾病大鼠肾组织ET-1、NO的影响,探讨大黄虫丸对肾小管间质微血管的影响及其作用机制。方法将雌性SD大鼠50只随机分为假手术组、模型组、缬沙坦组、大黄虫丸中剂量组、大黄虫丸高剂量组,采用单侧输尿管结扎的方法建立肾小管间质纤维化大鼠模型,应用生化、放射免疫法、病理形态学等方法观察大鼠肾功能及梗阻侧肾组织ET-1、NO的水平。结果模型组大鼠血尿素氮(BUN)、血肌酐(Scr),肾组织ET-1、NO及ET-1/NO水平较假手术组显著上升,各给药组大鼠BUN、Scr、ET-1及大黄虫丸高剂量组ET-1/NO水平明显低于模型组。结论大黄虫丸可能通过下调ET-1及ET-1/NO水平,改善缩血管和舒血管物质间的失衡,进而增加肾组织局部的血流量,减轻组织缺血及缺氧程度,延缓肾小管间质纤维化。
Objective To observe the effect of Dahuang Zhechong pill on ET-1 and NO in renal tissue of rats with obstructive nephropathy and to explore the effect of Dahuang Zhechong pill on tubulointerstitial microvascular and its mechanism. Methods Fifty female Sprague-Dawley rats were randomly divided into sham operation group, model group, valsartan group, rhubarb group, middle-dose rhubarb group and rhubarb group. The unilateral ureteral ligation was used to establish the inter- The rat model of renal fibrosis was established. The renal function and the level of ET-1 and NO in obstructed kidney were observed by biochemistry, radioimmunoassay and pathomorphology. Results The levels of BUN, Scr, ET-1, NO and ET-1 / NO in the model group were significantly higher than those in the sham-operated group -1 and rhubarb Zhechong pill high dose ET-1 / NO levels were significantly lower than the model group. Conclusion Dahuang Zhachong Pills may reduce the imbalance between vasoconstrictor and vasodilator substances by down-regulating the levels of ET-1 and ET-1 / NO, and then increase the local blood flow of renal tissue, reduce the degree of tissue ischemia and hypoxia, Tubulointerstitial fibrosis.