目的构建重组表皮生长因子受体(EGFR)噬菌体疫苗,并观察其抗肿瘤效果。方法将鸡源EGFR膜外部分的5个基因片段插入T7噬菌体表达系统中,EGFR蛋白以融合蛋白的形式表达在噬菌体的外壳蛋白10B上,从而构建成噬菌体疫苗。Western blot方法检测噬菌体壳蛋白的EGFR表达,ELISA方法检测免疫小鼠血清中抗EGFR抗体。分离免疫小鼠脾细胞,检测淋巴细胞对靶细胞A431的细胞毒作用。C57BL／6J小鼠经过4次免疫,接种Lewis肺癌细胞,记录肿瘤的生长情况,评价疫苗的抗肿瘤效果。结果成功构建5种鸡源EGFR噬菌体疫苗REPV-C1、REPV-C2、REPV-C3、REF-C4和REPV-C5。Western blot结果显示,噬菌体壳蛋白上有EGFR表达。经免疫的小鼠血清中可检测到抗EGFR抗体。免疫后的小鼠脾细胞对EGFR受体高表达的A431细胞具有杀伤作用,效靶比为10:1时,最大杀伤率可达45．74％±7．21％。肿瘤生长曲线显示,免疫后的C57BL／6J小鼠Lewis肺癌生长受到明显抑制。结论重组EGFR噬菌体疫苗具有抗肿瘤效果,利用噬菌体表达技术构建噬菌体疫苗是一种有效的抗肿瘤方法。 Objective To construct a recombinant epidermal growth factor receptor (EGFR) phage vaccine and observe its anti-tumor effect. Methods The gene fragment of 5 outer segments of chicken EGFR was inserted into the expression system of T7 phage. The EGFR protein was expressed on the coat protein 10B of the phage as a fusion protein to construct a bacteriophage vaccine. The EGFR expression of phage coat protein was detected by Western blot and anti-EGFR antibody in sera of immunized mice by ELISA. The splenocytes of immunized mice were isolated and the cytotoxic effect of lymphocytes on target cells A431 was tested. C57BL / 6J mice were immunized 4 times and inoculated with Lewis lung cancer cells, the growth of the tumor was recorded and the anti-tumor effect of the vaccine was evaluated. Results Five chicken-derived EGFR phage vaccines, REPV-C1, REPV-C2, REPV-C3, REF-C4 and REPV-C5 were successfully constructed. Western blot results showed that EGFR was expressed on the phage coat protein. Anti-EGFR antibodies can be detected in the immunized mouse sera. The immunized mouse spleen cells have a killing effect on A431 cells with high expression of EGFR receptor, and the maximal killing rate can reach 45.74% ± 7.21% when the target ratio is 10: 1. Tumor growth curve showed that the growth of Lewis lung carcinoma in C57BL / 6J mice after immunization was significantly inhibited. Conclusion Recombinant EGFR phage vaccine has anti-tumor effect. Phage vaccine expression using phage display is an effective anti-tumor method.