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目的 探讨微卫星不稳定性 (MSI)与胃癌的关系。方法 采用PCR聚丙烯酰胺凝胶电泳及银染技术 ,检测 30例胃癌肿瘤组织DNAD2S12 3位点及D3S10 6 7位点的MSI。结果 30例胃癌 2个位点MSI检出率分别为 :D2S12 3位点2 6 .7%(8/30 ) ,D3S10 6 7位点 33.3%(10 /30 )。结论 MSI是胃癌发生过程中的分子标志 ,在胃癌的发生中起着重要作用
Objective To investigate the relationship between microsatellite instability (MSI) and gastric cancer. Methods PCR-polyacrylamide gel electrophoresis and silver staining techniques were used to detect the MSI of DNAD2S12 3 sites and D3S10 67 sites in 30 cases of gastric cancer tissues. Results The detection rates of MSI in 30 patients with gastric cancer were: D2S12 3 sites 26.7% (8/30), and D3S10 67 sites 33.3% (10/30). Conclusion MSI is a molecular marker in the development of gastric cancer and plays an important role in the development of gastric cancer.