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以猪粪和秸秆为主要试验材料,添加不同浓度重金属Zn,采取发酵罐处理方法,在好氧高温条件下研究了重金属Zn对猪粪堆肥过程中多酚氧化酶、脱氢酶活性的变化,以及堆腐过程堆体温度、堆料pH值、胡敏酸E4/E6值的变化。结果表明:(1)低量重金属Zn处理(L)较不添加重金属Zn(CK)和添加高量重金属Zn(H)堆料升温快、温度高、高温持续时间长。(2)重金属Zn的加入对堆料的pH值影响不大,不是影响堆肥进程的直接原因。(3)H处理在整个堆肥过程中E4/E6值均高于L和CK,表明高浓度Zn处理抑制腐殖质的缩合和芳构化。(4)L处理的多酚氧化酶活性大多数时间高于H处理的活性,说明低量重金属Zn更好地促进了木质素的降解及其产物的转化。(5)从整个堆肥过程来看,3个不同处理的脱氢酶活性表现出一定的不稳定性,可能是重金属对脱氢酶活性有抑制作用的同时发生“抗性酶活性”现象。
Pig manure and straw as the main test material, adding different concentrations of heavy metal Zn, take fermenter treatment method under aerobic high temperature conditions of heavy metal Zn pig manure composting process of polyphenol oxidase, dehydrogenase activity changes, As well as the pile body temperature, heap pH value, humic acid E4 / E6 value changes. The results showed that: (1) The treatment with low amount of heavy metal Zn had faster heating, higher temperature and longer duration at high temperature than that without addition of heavy metal Zn (H) and heavy metal Zn (H). (2) The addition of heavy metal Zn has little effect on the pH value of the pile, and is not the direct reason that affects the compost process. (3) The values of E4 / E6 were higher than L and CK during the whole composting process by H treatment, indicating that high concentration of Zn inhibited the condensation and aromatization of humus. (4) The activity of L-treated polyphenol oxidase was higher than that of H treatment for most of the time, indicating that the low content of heavy metal Zn promoted the degradation of lignin and the conversion of its product better. (5) From the whole composting process, the dehydrogenase activities of the three different treatments showed some instability, which may be caused by the simultaneous occurrence of “resistant enzyme activity” by heavy metals while inhibiting the dehydrogenase activity .