论文部分内容阅读
目的检测慢性乙型肝炎患者HBV特异性CTL的CD28表达情况。方法利用MHC-Ⅰ-肽五聚体(PENTAM-ER)技术结合流式多色分析技术,直接离体情况下(DIRECTEXVIVO)检测慢性乙型肝炎患者3个HLA-A2限制性的HBV表位特异性CTL表达CD28的情况。结果在22例HLA-A2+患者中,检测出HBCAG(18~27)特异性CTL有13例,频率为0.05%~0.16%;CD28+的HBCAG(18~27)特异性CTL的百分比为40%(23%~61%)。检测出HBEAG(335~343)有8例,频率为0.05%~0.24%;CD28+的HBEAG(335~343)特异性CTL的百分比为43%(36%~60%)。检测出HBP(575~583)特异性CTL有9例,频率为0.05%~0.19%;CD28+的HBPAG(575~583)特异性CTL的百分比为61%(45%~70%)。结论不同HBV抗原表位特异性CTL的CD28表达情况不同。
Objective To detect the expression of CD28 in HBV-specific CTLs in patients with chronic hepatitis B Methods Three HLA-A2-restricted HBV epitopes in patients with chronic hepatitis B were detected by DIRECTEXVIVO assay using MHC-I-peptide pentamer (PENTAM-ER) combined with flow cytometry Sex CTLs express CD28. Results Of the 22 cases of HLA-A2 + patients, 13 cases of HBCAG (18-27) specific CTL were detected with a frequency of 0.05% -0.16%; the percentage of HBCAG (18-27) specific CTL of CD28 + was 40% 23% ~ 61%). Eight cases of HBEAG (335-343) were detected with a frequency of 0.05% -0.24%. The percentage of HBEAG (335-343) specific CTL of CD28 + was 43% (36% -60%). Nine specific CTLs of HBP (575 ~ 583) were detected at a frequency of 0.05% ~ 0.19%. The percentage of specific CTLs of HBPAG (575 ~ 583) in CD28 + cells was 61% (45% ~ 70%). Conclusion The expression of CD28 in different CTLs with different HBV epitopes is different.